A novel European H5N8 influenza A virus has increased virulence in ducks but low zoonotic potential

Emerging Microbes & Infections
Christian GrundMartin Beer

Abstract

We investigated in a unique setup of animal models and a human lung explant culture biological properties, including zoonotic potential, of a representative 2016 highly pathogenic avian influenza virus (HPAIV) H5N8, clade 2.3.4.4 group B (H5N8B), that spread rapidly in a huge and ongoing outbreak series in Europe and caused high mortality in waterfowl and domestic birds. HPAIV H5N8B showed increased virulence with rapid onset of severe disease and mortality in Pekin ducks due to pronounced neuro- and hepatotropism. Cross-species infection was evaluated in mice, ferrets, and in a human lung explant culture model. While the H5N8B isolate was highly virulent for Balb/c mice, virulence and transmissibility were grossly reduced in ferrets, which was mirrored by marginal replication in human lung cultures infected ex vivo. Our data indicate that the 2016 HPAIV H5N8B is avian-adapted with augmented virulence for waterfowl, but has low zoonotic potential. The here tested combination of animal studies with the inoculation of human explants provides a promising future workflow to evaluate zoonotic potential, mammalian replication competence and avian virulence of HPAIV.

References

May 1, 1980·Canadian Journal of Microbiology·V S HinshawB Turner
Apr 14, 2004·Journal of Virology·Katharine M Sturm-RamirezRobert G Webster
Aug 17, 2005·Journal of Virology·K M Sturm-RamirezR G Webster
Jun 8, 2007·Emerging Infectious Diseases·Steven L SalzbergIlaria Capua
Mar 2, 2010·The Journal of Infectious Diseases·Richard J Whitley
Aug 4, 2011·Disease Models & Mechanisms·Jessica A BelserTerrence M Tumpey
Jan 19, 2012·Human Vaccines & Immunotherapeutics·Leslie A ReperantAlbert D M E Osterhaus
Jul 26, 2012·The Journal of Infectious Diseases·Viola K WeinheimerAndreas C Hocke
Mar 6, 2013·Virus Research·Jessica A Belser, Terrence M Tumpey
Oct 3, 2013·American Journal of Respiratory and Critical Care Medicine·Andreas C HockeThorsten Wolff
Apr 9, 2014·Journal of Immunological Methods·Rajagowthamee R Thangavel, Nicole M Bouvier
May 27, 2014·Emerging Infectious Diseases·Youn-Jeong LeeHee-Soo Lee
Jul 31, 2014·Emerging Infectious Diseases·Haibo WuNanping Wu
Jan 28, 2015·Emerging Infectious Diseases·Hyun-Mi KangYoun-Jeong Lee
Feb 7, 2015·Science·Josanne H VerhagenRon A M Fouchier
May 13, 2015·Influenza and Other Respiratory Viruses·Gavin J D SmithUNKNOWN World Health Organization/World Organisation for Animal Health/Food and Agriculture Organization (WHO/OIE/FAO) H5 Evolution
Jun 4, 2015·Emerging Microbes & Infections·Young-Il KimYoung Ki Choi
Jan 28, 2016·Avian Pathology : Journal of the W.V.P.A·Dong-Hun LeeChang-Seon Song
Aug 26, 2016·Journal of Virology·Mary J Pantin-JackwoodDavid E Swayne
Nov 23, 2016·Emerging Infectious Diseases·Dong-Hun LeeAlexander Shestopalov
Feb 9, 2017·Emerging Infectious Diseases·Mingxin LiJianjun Chen
May 19, 2017·Emerging Infectious Diseases·Abdullah A SelimMahmoud M Naguib

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Citations

Jan 24, 2020·Emerging Microbes & Infections·Susanne KoetheAnja Globig
Mar 7, 2020·Reviews in Medical Virology·Reina YamajiWenqing Zhang
Sep 19, 2019·Nature Microbiology·Kevin CiminskiMartin Schwemmle
May 6, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Samantha J LycettPaul Digard
Aug 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Samantha J LycettUNKNOWN Global Consortium for H5N8 and Related Influenza Viruses
Mar 8, 2020·Viruses·Jacqueline KingAnne Pohlmann
Nov 20, 2019·Emerging Infectious Diseases·Dai-Lun ShinGeorg Herrler
Sep 27, 2018·EFSA Journal·UNKNOWN European Food Safety AuthorityFrancesca Baldinelli
Jul 28, 2020·Scientific Reports·Peter H F HobbelenThomas J Hagenaars
Apr 10, 2020·Avian Pathology : Journal of the W.V.P.A·Rokshana ParvinTimm Harder

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Methods Mentioned

BETA
ELISA

Related Concepts