Abstract
The development of models that incorporate intact microvascular networks enables the investigation of multicellular dynamics during angiogenesis. Our laboratory introduced the rat mesentery culture model as such a tool, which would be enhanced with mouse tissue. Since mouse mesentery is avascular, an alternative is mouse mesometrium, the connective tissue of uterine horns. The study's objective was to demonstrate that mouse mesometrium contains microvascular networks that can be cultured to investigate multicellular dynamics during angiogenesis. Harvested mesometrium tissues from C57Bl/6 female mice were cultured in media with serum for up to 7 days. PECAM, NG2, αSMA, and LYVE-1 labeling identified endothelial cells, pericytes, smooth muscle cells, and lymphatic endothelial cells, respectively. These cells comprised microvascular networks with arterioles, venules, and capillaries. Compared to day 0, capillary sprouts per vascular length were increased by 3 and 5 days in culture (day 0, 0.08 ± 0.01; day 3, 3.19 ± 0.78; day 5, 2.49 ± 0.05 sprouts/mm; p < 0.05). Time-lapse imaging of cultured tissues from FlkEGFP mice showcases the use of the model for lineage studies. The impact is supported by the identification of endothelial c...Continue Reading
References
Jan 22, 2003·Microvascular Research·Ana M Ponce, Richard J Price
Apr 13, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Walter L MurfeeShayn M Peirce
Dec 16, 2005·Nature·Peter Carmeliet
Apr 22, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Walter L MurfeeThomas C Skalak
Sep 23, 2006·Journal of Cellular and Molecular Medicine·K Norrby
May 25, 2007·Nature Reviews. Molecular Cell Biology·Ralf H Adams, Kari Alitalo
Jul 17, 2007·Microvascular Research·Anne M Goodwin
Dec 11, 2007·Cardiovascular Research·Andrew V BenestDavid O Bates
Sep 6, 2008·The American Journal of Pathology·Christopher R AndersonRichard J Price
Jul 22, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jennifer L RobichauxWalter L Murfee
Sep 28, 2010·Nature Cell Biology·Lars JakobssonHolger Gerhardt
Jun 2, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Ming YangWalter L Murfee
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jonathan W Song, Lance L Munn
Oct 1, 2011·Microvascular Research·Peter C Stapor, Walter L Murfee
Jun 23, 2012·BMC Physiology·Molly R Kelly-GossWalter L Murfee
Nov 6, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Peter C StaporWalter L Murfee
Dec 18, 2012·Lymphatic Research and Biology·Richard S SweatWalter L Murfee
May 31, 2013·Frontiers in Physiology·Molly R Kelly-GossWalter L Murfee
Nov 26, 2013·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Molly R Kelly-GossWalter L Murfee
Mar 25, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Richard S SweatWalter L Murfee
May 24, 2014·Journal of Vascular Research·Peter C StaporWalter Lee Murfee
Mar 6, 2015·PloS One·Mohammad S AzimiWalter L Murfee
May 3, 2015·Biomaterials·Peter DelNeroClaudia Fischbach
Nov 20, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Richard S SweatWalter L Murfee
May 2, 2017·Nature Neuroscience·Neil I BowerBenjamin M Hogan
May 21, 2017·Scientific Reports·Jessica M MotherwellWalter L Murfee