A novel external esophageal perfusion model for reflux esophageal injury.

Digestive Diseases and Sciences
Yan LiRobert C G Martin

Abstract

The current animal models of esophagitis and Barrett's esophagus consist of surgeries that divert the gastroduodenal contents to the esophagus. The limitations of these models are the inability to control the amount and concentration of the refluxate and the causing of significant postoperative stress and morbidity. Eighteen adult rats were cannulated at the upper esophagus and connected to a subcutaneous osmotic micropump to perfuse the esophageal lumen with bile and acid. Animals were sacrificed after 7 days of perfusion. Histological changes were determined. Cell proliferation, apoptosis, lipid peroxidation, and glutathione were measured. Histopathological changes in the bile- or acid-perfused esophagus were consistent with the findings associated with reflux esophagitis. Enhanced proliferation and apoptosis were seen, along with increased oxidative stress. The external esophageal perfusion model enabled precise control of the injurious agent. It induced the histologic and cellular injury of reflux esophagitis after 7 days.

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Citations

Nov 20, 2008·Annals of Surgical Oncology·Yan LiRobert C G Martin
Jul 3, 2013·International Journal of Nanomedicine·Yan LiRobert C G Martin
Sep 26, 2013·Journal of Clinical Biochemistry and Nutrition·Masato TamuraIchinosuke Hyodo
Oct 14, 2009·World Journal of Gastroenterology : WJG·Sae-Kwang KuSeong-Soo Roh
Aug 15, 2014·Journal of Clinical Biochemistry and Nutrition·Masato TamuraIchinosuke Hyodo
Jul 28, 2015·Clinical and Translational Science·Harit KapoorSumeet K Mittal
Jun 13, 2008·European Journal of Pharmacology·Chandana Venkateswara Rao, Madhavan Vijayakumar
Jul 20, 2010·The Journal of Surgical Research·Suzanne C SchiffmanRobert C G Martin
Sep 1, 2007·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·Yan Li, Robert C G Martin
Jul 23, 2009·Molecular Carcinogenesis·Yan LiRobert C G Martin
May 9, 2015·World Journal of Gastroenterology : WJG·Rui-Hua Wang
Jan 18, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Yue QiuKe-Feng Ruan
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert C G MartinYan Li

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