A novel fabrication method to create a thick collagen bundle composed of uniaxially aligned fibrils: an essential technology for the development of artificial tendon/ligament matrices

Journal of Biomedical Materials Research. Part a
Shunji YunokiKazunori Yasuda

Abstract

In this study, we developed a fabrication method for thick collagen gel bundles comprising uniaxially aligned fibrils of sufficient size for filling defects in ligament tissues. The fabrication involved rotary shearing to dense collagen sols using a rheometer and then warming them from 23°C to 37°C to trigger gelation upon rotation. Gelation due to collagen fibril formation was accelerated by increased concentrations of neutral phosphate buffer, and fibril alignment occurred within 20 s during the early stage of rapid gelation. Fabrication of gels was completed with slippage between gels and the movable upper plate, and well-aligned fibrils along the rotation direction were observed in the marginal regions of disc-shaped gels. Gel thickness could be increased from 1 to 3 mm with homogeneous alignment of fibrils in the entire sample. The alignment of fibrils improved mechanical properties against tensile loads that were placed parallel to the alignment axis. Elongation of cultured fibroblast along the alignment was observed on the gels. The present method will enable the bottom-up fabrication of an artificial tendon for ligament reconstruction and repair.

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Citations

Aug 16, 2016·Materials Science & Engineering. C, Materials for Biological Applications·M B FauziS R Chowdhury
Sep 11, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shunji YunokiKazunori Yasuda
Nov 30, 2018·Current Drug Targets·Irene Anton-SalesDana Kralisch
Jun 12, 2020·Tissue Engineering. Part a·Ortal ShelahAyelet Lesman
May 20, 2018·Physical Review. E·A Sengab, R C Picu
Apr 27, 2018·Journal of the Royal Society, Interface·Mathew BaldwinAndrew Carr
Jun 30, 2017·Tissue Engineering and Regenerative Medicine·Guang-Zhen Jin, Hae-Won Kim
Aug 15, 2020·Digestive Endoscopy : Official Journal of the Japan Gastroenterological Endoscopy Society·Toshio UraokaMasayuki Shimoda
Feb 23, 2021·Digestive Endoscopy : Official Journal of the Japan Gastroenterological Endoscopy Society·Toshio UraokaNaohisa Yahagi

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