A Novel Fat Making Strategy for Adipose-Derived Progenitor Cells Enrichment Fat Improves Fat Graft Survival.

Aesthetic Surgery Journal
Pengju FanShaorong Lei

Abstract

A low survival rate is one of the main challenges in fat grafting. This study aimed to evaluate whether the microfat obtained using a novel strategy promoted the survival and retention of fat grafts. A 5 mm diameter blunt tip cannula with larger side holes (~30 mm 2 per hole) was used to obtain macrofat. Subsequently, a novel strategy based on a newly invented extracorporeal cutting device was used to cut the macrofat into microfat, which was named adipose-derived progenitor cells enrichment fat (AER fat); Coleman fat was used as the control. Aliquots (0.5 mL) of both groups of fat were transplanted into 10 nude mice and analyzed 10 weeks later. Western blotting, flow cytometry, and immunofluorescence were performed to assess the AER characteristics and underlying mechanisms. The retention rate of fat grafts in AER fat-treated animals was significantly higher than that in the Coleman group (54.6±13% vs. 34.8±9%, P<0.05) after 10 weeks. AER fat contained more DPP4 + progenitor cells (3.3±0.61×10 3 vs. 2.0±0.46×10 3 cells/mL, P<0.05), adipose-derived plastic-adherent cells (6.0±1.10×10 4 vs. 2.6±0.17×10 4 cells/mL, P<0.001), and viable adipocytes as compared to Coleman fat. Moreover, histological analysis showed that AER fat graf...Continue Reading

References

Aug 29, 2006·Plastic and Reconstructive Surgery·Sydney R Coleman
Jun 19, 2007·Plastic and Reconstructive Surgery·Andrea Moreira GonzalezIan T Jackson
Jun 24, 2008·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Melike ErdimAydin Sav
Dec 17, 2008·Aesthetic Surgery Journal·Joan FontdevilaEsteban Martinez
May 1, 2006·Aesthetic Surgery Journal·Zafer OzsoyAyhan Bilir
Nov 26, 2009·Plastic and Reconstructive Surgery·Hitomi EtoKotaro Yoshimura
Dec 3, 2010·Plastic and Reconstructive Surgery·Hirotaka SugaKotaro Yoshimura
Jan 3, 2012·Expert Opinion on Biological Therapy·Brian J PhilipsJ Peter Rubin
Jan 21, 2012·Plastic and Reconstructive Surgery·Hitomi EtoKotaro Yoshimura
Mar 21, 2015·Aesthetic Surgery Journal·Tejaswi IyyankiQixu Zhang
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Mar 29, 2019·Aesthetic Plastic Surgery·Xiaoxuan LeiBiao Cheng

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