A novel feature of the multistep phosphorelay in Escherichia coli: a revised model of the RcsC --> YojN --> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour

Molecular Microbiology
S TakedaT Mizuno

Abstract

In this study, we re-investigated the previously characterized RcsC (sensor His-kinase) --> RcsB (response regulator) phosphorelay system that is involved in the regulation of capsular polysaccharide synthesis in Escherichia coli. The previously proposed model hypothesized the occurrence of a direct phosphotransfer from RcsC to RcsB in response to an unknown external stimulus. As judged from the current general view as to the His --> Asp phosphorelay, this RcsC --> RcsB framework is somewhat puzzling, because RcsC appears to contain both a His-kinase domain and a receiver domain, but not a histidine (His)-containing phosphotransmitter domain (e.g. HPt domain). We thus suspected that an as yet unknown mechanism might be underlying in this particular His --> Asp phosphorelay system. Here, we provide several lines of in vivo and in vitro evidence that a novel and unique His-containing phosphotransmitter (named YojN) is essential for this signalling system. A revised model is proposed in which the multistep RcsC --> YojN --> RcsB phosphorelay is implicated. It was also demonstrated that this complex signalling system is somehow involved in the modulation of a characteristic behaviour of E. coli cells during colony formation on the ...Continue Reading

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Citations

Jun 16, 2007·Applied Microbiology and Biotechnology·Weiwen ZhangJohannes C M Scholten
Jan 10, 2003·Molecular Microbiology·Chakib Mouslim, Eduardo A Groisman
Jun 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yi-Cheng SunCreg Darby
Sep 23, 2011·Molecular Plant-microbe Interactions : MPMI·Dongping WangYoufu Zhao
Nov 11, 2008·Applied and Environmental Microbiology·Michael Ionescu, Shimshon Belkin
Jun 3, 2006·Journal of Bacteriology·Marie-Pierre Castanié-CornetAnnick Jacq
Apr 29, 2008·Journal of Bacteriology·Elizabeth A HussaKaren L Visick
May 15, 2012·Journal of Bacteriology·Cristina LatasaIñigo Lasa
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Jan 15, 2008·Journal of Bacteriology·Janet K Hatt, Philip N Rather
Nov 13, 2007·Journal of Bacteriology·Xingqi ShiLotte Søgaard-Andersen
Feb 17, 2009·Journal of Bacteriology·Clara B García-CalderónFrancisco Ramos-Morales
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Aug 24, 2004·Bioscience, Biotechnology, and Biochemistry·Daisuke HagiwaraTakeshi Mizuno

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