PMID: 9429091Jan 16, 1998Paper

A novel fiber-optic pH sensor incorporating carboxy SNAFL-2 and fluorescent wavelength-ratiometric detection

Journal of Biomedical Materials Research
Z XuR E Marchant

Abstract

A fiber-optic pH sensor was prepared using the self-referencing fluorescent pH probe carboxy seminaphtho-fluorescein (SNAFL-2). The ratio of the emission from the base form of this dye and the emission near the isoemissive point is insensitive to variations in the excitation intensity and photobleaching. The carboxy SNAFL-2 and a photoaffinity crosslinker, 4-azido-2,3,5,6-tetrafluorobenzoic acid, succinimidyl ester, were attached onto poly(acrylamide-co-vinylamine) to form a hydrophilic functional membrane for the fiber-optic sensor. Photo crosslinking was used to create a crosslinked pH-sensing membrane and covalently bind the membrane onto the surface of the PMMA optical fiber. The fluorescent properties of the membrane-fiber conjugate have been determined. The membrane is stable, and the pH sensor shows a fast response time and excellent resolution in a wide pH range of 3 to 11.

References

May 1, 1991·Analytical Biochemistry·J E WhitakerF G Prendergast
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Apr 1, 1993·Analytical Chemistry·Richard B Thompson, Joseph R Lakowicz
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Citations

Jan 25, 2011·Analytical and Bioanalytical Chemistry·Daniel AignerIngo Klimant
Feb 6, 2009·Biotechnology Progress·Ramachandram BaduguLeah Tolosa
Sep 1, 2005·Analytical and Bioanalytical Chemistry·Cheng-Gang NiuPin-Zhu Qin
Apr 10, 2014·Methods and Applications in Fluorescence·Susanne SchuttingSergey M Borisov
Jan 3, 2001·Biotechnology and Bioengineering·Y KostovG Rao
Nov 5, 2020·Chemical Reviews·Andreas SteineggerSergey M Borisov

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