PMID: 9442101Feb 28, 1998Paper

A novel fission yeast gene, tht1+, is required for the fusion of nuclear envelopes during karyogamy

The Journal of Cell Biology
Y TangeO Niwa

Abstract

We have isolated a fission yeast karyogamy mutant, tht1, in which nuclear congression and the association of two spindle pole bodies occurs but the subsequent fusion of nuclear envelopes is blocked. The tht1 mutation does not prevent meiosis, so cells execute meiosis with two unfused nuclei, leading to the production of aberrant asci. The tht1(+) gene was cloned and sequenced. Predicted amino acid sequence has no significant homology to previously known proteins but strongly suggests that it is a type I membrane protein. The tht1(+) gene is dispensable for vegetative growth and expressed only in conjugating cells. Tht1p is a glycoprotein susceptible to endoglycosilase H digestion. Site- directed mutagenesis showed that the N-glycosylation site, as well as the COOH-terminal region of Tht1p, is essential for its function. A protease protection assay indicated that the COOH terminus is cytoplasmic. Immunocytological analysis using a HA-tagged Tht1p suggested that the protein is localized in nuclear envelopes and in the ER during karyogamy and that its levels are reduced in cells containing fused nuclei.

References

Aug 1, 1992·Current Opinion in Cell Biology·S High, B Dobberstein
Jan 2, 1992·Nature·M J Gething, J Sambrook
Jan 1, 1991·Annual Review of Microbiology·M D Rose
Feb 1, 1991·Molecular and Cellular Biology·S SilveR Haguenauer-Tsapis
Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D BakerN Segev
Dec 1, 1989·The Journal of Cell Biology·T G Chappell, G Warren
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Oct 25, 1995·Molecular & General Genetics : MGG·Y Tange, O Niwa
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·J L BrodskyR Schekman
Aug 1, 1994·The Journal of Cell Biology·L J KuriharaM D Rose
Apr 8, 1994·Science·Y ChikashigeY Hiraoka
Feb 1, 1996·The Journal of Cell Biology·D T Ng, P Walter
Aug 1, 1996·Molecular and Cellular Biology·L J KuriharaM D Rose
Sep 1, 1996·Molecular Microbiology·C GarnierR Haguenauer-Tsapis
May 16, 1997·The Journal of Biological Chemistry·S Nishikawa, T Endo
Jan 1, 1985·Molecular & General Genetics : MGG·Y Lino, M Yamamoto

❮ Previous
Next ❯

Citations

Jun 1, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Yamamoto, Y Hiraoka
Jun 1, 2005·Trends in Biotechnology·Graham LaddsJohn Davey
Apr 12, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·D Q DingY Hiraoka
May 18, 2007·FEMS Yeast Research·Laura Ongay-LariosRoberto Coria
May 19, 2007·Cell Division·Ayami OhtakaHiroshi Nojima
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Juan Mata, Jürg Bähler
Sep 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Christelle VasnierEric Espagne
Nov 24, 2005·Current Biology : CB·Cristina Martín-CastellanosSergio Moreno
Nov 7, 2009·Current Opinion in Microbiology·Racquel K Sherwood, Richard J Bennett
Jan 13, 1999·Yeast·J Davey
Nov 20, 2003·Journal of Cell Science·Meredith Johnson SagollaW Zacheus Cande
Nov 9, 2021·The Journal of Cell Biology·Qian ZhuXiangwei He

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.