A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings

Microbiological Research
Peter F DuckworthSarah E Maddocks

Abstract

Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments. Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface. In this manuscript, a new biofilm flow device for study of wound biofilms is reported. The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings. Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8-10 colony forming units (CFU). There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.

Citations

Jan 10, 2021·Advances in Colloid and Interface Science·Milos KrsmanovicAndrew K Dickerson
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Andi Dian PermanaRyan F Donnelly
Feb 13, 2021·Molecular Microbiology·Deborah Bow Yue YungDaniel Pletzer
Mar 3, 2020·Current Drug Targets·Sandeep K ShuklaPriyanka Shaw
Feb 23, 2020·Journal of Microbiological Methods·Mathilde Frost KristensenSebastian Schlafer
Nov 23, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Naila Bou HaidarAnthony C Duncan
Jul 29, 2021·Journal of Applied Microbiology·Andreea-Gabriela NedeleaSarah E Maddocks

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