A novel fluorescence-based array biosensor: principle and application to DNA hybridization assays

Biosensors & Bioelectronics
E SchultzF Perraut

Abstract

A novel fluorescence-based array biosensor targeted for field applications, such as environmental monitoring, has been developed, and successfully applied to DNA hybridization assays. The purpose was to meet the demand for automated, portable but easy-to-maintain systems allowing continuous flow monitoring of surface reactions. The biosensor presented here can be distinguished from the existing systems by the optical method used, which provides an enhanced simplicity and robustness, and enables a simple maintenance by potentially unskilled personnel. The system is based on a conventional microscope slide which acts both as transducer and biological array sensor. The excited fluorescence is guided by total internal reflection into the slide to the detector which is directly interfaced to the slide. Each region of the sensor array is successively optically interrogated, and the detection of the corresponding fluorescent emission synchronized. A real-time three-analyte analysis is thus feasible without any mechanical scanning movement or optical imaging systems as generally used in the existing instruments. The ability of the biosensor to operate in continuous flow for several tens of hours has been demonstrated. The biosensor has...Continue Reading

References

Nov 18, 2004·Journal of Lipid Research·Marie-Thérèse BerthierMarie-Claude Vohl

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Citations

Apr 2, 2010·Archives of Microbiology·M Z AbdinSaleem Javed
May 9, 2012·Biosensors & Bioelectronics·Cyril DelattreChristine Peponnet
Feb 18, 2010·Electrophoresis·Zachary GagnonHsueh-Chia Chang
May 13, 2009·Analytical Chemistry·Kadir AslanChris D Geddes

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