A novel fluorescent aptasensor based on gold and silica nanoparticles for the ultrasensitive detection of ochratoxin A

Nanoscale
Seyed Mohammad TaghdisiKhalil Abnous

Abstract

Analytical approaches for the detection and quantitation of ochratoxin A (OTA) in blood serum and food products are high in demand. In this study, a fluorescent aptamer-based sensor (aptasensor) is developed for the selective and sensitive detection of OTA, based on a complementary strand of aptamer (CS) and two types of nanoparticles, gold nanoparticles (AuNPs) and silica nanoparticles (SNPs) coated with streptavidin. The fabricated aptasensor inherits the characteristics of SNPs, as enhancers of fluorescence intensity; AuNPs, such as large surface area and unique optical properties; and high affinity of the aptamer toward its target compared to its CS. In the absence of OTA, no FAM and biotin-labeled CS is in the environment of the SNPs coated with streptavidin, which leads to no fluorescence emission. In the presence of the target, an FAM and biotin-labeled CS-SNPs coated with streptavidin conjugate is formed, thus resulting in a very strong fluorescence emission. The designed fluorescent aptasensor exhibits high selectivity toward OTA with a limit of detection (LOD) as low as 0.098 nM. Furthermore, the fabricated aptasensor was successfully applied for the detection of OTA in grape juice and serum with LODs of 0.113 and 0.1...Continue Reading

References

Oct 16, 2002·Biomedical Chromatography : BMC·Hassan Y Aboul-EneinMuzaffer Tunçel
Aug 18, 2005·Journal of Agricultural and Food Chemistry·Feng-Yih YuChing-Chyuan Su
Mar 4, 2014·Critical Reviews in Food Science and Nutrition·Yiyang DongDaning Wang
Mar 5, 2014·Biosensors & Bioelectronics·Jinghua ChenJing Zhang
Apr 16, 2014·The Analyst·Wenhu ZhouJuewen Liu
Mar 1, 2015·Biosensors & Bioelectronics·Shimaa EissaMohammed Zourob
Mar 12, 2015·Toxins·Simona Marianna SanzaniAntonio Ippolito
Mar 31, 2015·Biosensors & Bioelectronics·Mohammad RamezaniSeyed Mohammad Taghdisi
Apr 9, 2015·Nanoscale Research Letters·Jamie BensonKar Seng Teng

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Citations

Jun 18, 2016·Analytical and Bioanalytical Chemistry·Morteza Alinezhad NameghiSeyed Mohammad Taghdisi
Jun 19, 2017·Analytical Biochemistry·Najmeh AnsariKiarash Ghazvini
Nov 17, 2017·Toxins·Amina RhouatiJean Louis Marty
Dec 14, 2018·Methods and Applications in Fluorescence·Sena Ghayyem, Farnoush Faridbod
Feb 23, 2020·Advanced Healthcare Materials·Yu MaYa Ding
Aug 30, 2020·Biosensors·Marc PranteJohanna Walter
Apr 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Helena FerreiraNuno M Neves
Jan 21, 2017·Analytical Chemistry·Yan Du, Shaojun Dong

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