PMID: 8605231Feb 9, 1996Paper

A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli

Biochimica Et Biophysica Acta
K YoshiokaHideaki Matsuoka

Abstract

A novel fluorescent derivative of glucose was synthesized by reacting D-glucosamine and NBD-Cl. The TLC analysis of the reaction mixture showed the generation of a single spot with intense fluorescence (lambda Ex = 475 nm, lambda Em = 550 nm). The obtained novel fluorescent product, which was identified as 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) by 1H-NMR and FAB-MS spectrometries, was applied to the assessment of the glucose uptake activity of Escherichia coli B. 2-NBDG accumulated in living cells and not in dead cells. The uptake of 2-NBDG was competitively inhibited by D-glucose and not by L-glucose, which suggested the involvement of the glucose transporting system in the uptake of 2-NBDG. 2-NBDG taken into the cytoplasma of E. coli cells was supposedly converted into another derivative in the glucose metabolic pathway.

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Citations

Aug 14, 2012·The Journal of Membrane Biology·Elham Raeisi, Lluis M Mir
Jul 11, 2006·Journal of Industrial Microbiology & Biotechnology·Kohtaro FujiokaHideaki Matsuoka
Jun 15, 2007·Journal of Industrial Microbiology & Biotechnology·Kohtaro FujiokaHideaki Matsuoka
Nov 2, 2007·Glycoconjugate Journal·M Carmen LouzaoLuis M Botana
Nov 15, 2005·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Roger G O'NeilNizar Mullani
Sep 3, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Mohamed HassaneinVito Quaranta
Oct 6, 2011·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Alexandru V KorotcovPaul C Wang
Sep 23, 2000·Journal of Microbiological Methods·A Natarajan, F Srienc
Jun 1, 2002·International Journal of Food Microbiology·Ki-Bong Oh, Hideaki Matsuoka
May 22, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F LeiraM R Vieytes
Sep 3, 2013·Nature Neuroscience·William A FlavahanAnita B Hjelmeland
Sep 27, 2013·Nature Neuroscience·Shirin Ilkhanizadeh, William A Weiss
Apr 6, 2012·Nature Reviews. Cancer·Benjamin J VakocBrett E Bouma
Apr 23, 2011·Diabetes Technology & Therapeutics·Amy B BlodgettNiloofar M Tabatabai
Apr 15, 2014·Journal of Physiology and Biochemistry·Xihong ZhouYizhen Wang
Dec 11, 2013·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Eduardo H MoriyamaBrian C Wilson
Aug 10, 2010·Journal of Photochemistry and Photobiology. B, Biology·An-Long HuHan-Hong Xu
Jun 30, 2015·Biological & Pharmaceutical Bulletin·Yuta MorishigeFumio Amano
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Oct 13, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·H SträuberS Müller
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Nov 14, 2015·Nature Communications·Shigenori MiuraShoji Takeuchi
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Jun 29, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Heike Sträuber, Susann Müller
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Aug 11, 2000·Metabolic Engineering·A Natarajan, F Srienc
Apr 2, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jim ZhongMark W Dewhirst
Oct 5, 2013·Biomaterials·Liqiang ZhangDeirdre Meldrum
Mar 23, 2013·FEMS Microbiology Letters·Mia RysselHenrik Siegumfeldt

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