A novel fluorescent nanosensor for detection of heparin and heparinase based on CuInS2 quantum dots

Biosensors & Bioelectronics
Ziping LiuXingguang Su

Abstract

In this work, a novel fluorescence "turn off-on" nanosensor for the determination of heparin and heparinase based on CuInS2 quantum dots (QDs) was established. CuInS2 QDs (modified by l-cysteine) featuring amino groups were directly prepared in aqueous solution via a hydrothermal synthesis method. The amino groups on the surface of CuInS2 QDs can interact with sulfate and carboxylate groups in heparin via electrostatic interactions and hydrogen bonding, which led the fluorescence of CuInS2 QDs to "turn-off". However, the heparin could be hydrolyzed into small fragments in the presence of heparinase, which resulted in the fluorescence of CuInS2 QDs being recovered. Therefore, the addition of heparinase to the heparin/CuInS2 QDs system activated the fluorescence of CuInS2 QDs to "turn-on" state. Thus, the determination of heparin and heparinase could be achieved by monitoring the fluorescence "turn off-on". Under the optimum conditions, there was a good linear relationship between I/I0 (I and I0 were the fluorescence intensity of CuInS2 QDs in the presence and absence of heparin, respectively) and heparin concentration in the range of 0.05-15 μmol L(-1) with the detection limit of 12.46 nmol L(-1). The linear detection for hepari...Continue Reading

References

Oct 1, 1992·Chest·J S Ginsberg, J Hirsh
Jan 24, 2007·Chembiochem : a European Journal of Chemical Biology·Jimena C SaucedaMark Nitz
Feb 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Sung Ju ChoFrançoise M Winnik
Oct 25, 2011·The Analyst·Qiang Ma, Xingguang Su

❮ Previous
Next ❯

Citations

Jan 14, 2016·The Journal of Physical Chemistry Letters·Alice D P Leach, Janet E Macdonald
Jul 26, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Shenghua LiaoZhengyu Yan
Nov 7, 2015·Luminescence : the Journal of Biological and Chemical Luminescence·B RezaeiAli A Ensafi
Jan 10, 2017·ACS Applied Materials & Interfaces·Long ZhaoXiaohong Li
Apr 11, 2017·Chemical Reviews·Claudia CoughlanKevin M Ryan
Mar 7, 2017·Chemical Communications : Chem Comm·Mingyao MouShenghua Liao
Dec 23, 2014·Chemical Communications : Chem Comm·Jean-Patrick FrancoiaLaurent Vial
Apr 18, 2018·Analytical and Bioanalytical Chemistry·Ziping Liu, Shasha Liu
Oct 13, 2020·Chemical Communications : Chem Comm·Gyan H AryalLiming Huang
Feb 24, 2021·International Journal of Biological Macromolecules·Shrishti P PandeyPrabhat K Singh
Mar 13, 2021·Analytical Methods : Advancing Methods and Applications·Ziang GuoXiaobo Zou
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Raybel MuñozJose A Rodriguez
Oct 31, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Anup Kumar GhoshPrasanta Kumar Das
Dec 10, 2016·ACS Applied Materials & Interfaces·Niyati H Mudliar, Prabhat K Singh
Nov 5, 2019·ACS Chemical Biology·Benjamin Ourri, Laurent Vial

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.