PMID: 9539722May 16, 1998Paper

A novel glutamine-RNA interaction identified by screening libraries in mammalian cells

Proceedings of the National Academy of Sciences of the United States of America
R Tan, A D Frankel

Abstract

The arginine-rich motif provides a versatile framework for RNA recognition in which few amino acids other than arginine are needed to mediate specific binding. Using a mammalian screening system based on transcriptional activation by HIV Tat, we identified novel arginine-rich peptides from combinatorial libraries that bind tightly to the Rev response element of HIV. Remarkably, a single glutamine, but not asparagine, within a stretch of polyarginine can mediate high-affinity binding. These results, together with the structure of a Rev peptide-Rev response element complex, suggest that the carboxamide groups of glutamine or asparagine are well-suited to hydrogen bond to G-A base pairs and begin to establish an RNA recognition code for the arginine-rich motif. The screening approach may provide a relatively general method for screening expression libraries in mammalian cells.

References

Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·N C SeemanA Rich
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M B FeinbergA D Frankel
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B Seed, A Aruffo
Jun 14, 1967·Journal of Molecular Biology·B Hirt
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W Schaffner
Oct 1, 1995·Current Opinion in Biotechnology·B Seed
Jun 4, 1993·Cell·R TanA D Frankel
Aug 5, 1993·Journal of Molecular Biology·M F LaspiaM B Mathews
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·R Tan, A D Frankel
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·I A Laird-Offringa, J G Belasco
Mar 14, 1996·Nature·K HaradaA D Frankel
Apr 15, 1996·Nucleic Acids Research·D E Fouts, D W Celander
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·D J SenGuptaM Wickens
Aug 1, 1996·Human Gene Therapy·T M Kinsella, G P Nolan
Mar 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·J E Wilhelm, R D Vale

❮ Previous
Next ❯

Citations

May 20, 1999·Biopolymers·M A Weiss, N Narayana
Mar 26, 2003·Journal of Molecular Biology·Alan C ChengAlan D Frankel
Aug 10, 2001·Current Opinion in Structural Biology·A C ChengA D Frankel
Mar 16, 2006·Journal of the American Chemical Society·Nicholas L MillsR Kiplin Guy
Aug 12, 1998·Nature Structural Biology·I A Laird-Offringa, J G Belasco
Feb 28, 2001·The EMBO Journal·D M CampisiA D Frankel
Apr 3, 2001·Molecular and Cellular Biology·A Venkatesan, A Dasgupta
Jul 27, 2005·RNA·Tetsuya IwazakiKazuo Harada
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·D J McCollA D Frankel
Aug 26, 2014·FEBS Letters·Camilla Faoro, Sandro F Ataide
Jul 2, 2015·Journal of Molecular Recognition : JMR·Emane Y Abdallah, Colin A Smith
Mar 21, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Maki SugayaKazuo Harada
Apr 7, 2016·Cell Communication and Signaling : CCS·Aino I JärvelinAlfredo Castello
Mar 17, 2015·Biochemical and Biophysical Research Communications·Yuuki FujiwaraTomohiro Kabuta
Dec 7, 2000·Molecular Cell·C A SmithA D Frankel
Aug 2, 2003·Chemistry & Biology·Coby B CarlsonPeter A Beal
Aug 20, 2003·Biopolymers·Chandreyee Das, Alan D Frankel
Apr 19, 2017·Antimicrobial Agents and Chemotherapy·Robert L NakamuraAdam R Renslo
Sep 1, 2006·Current Opinion in HIV and AIDS·Dirk Daelemans, Christophe Pannecouque
Jan 11, 2020·Journal of Molecular Recognition : JMR·Nicole G RaadColin A Smith
Jan 7, 2017·Virus Evolution·Ryan Ruboyianes, Michael Worobey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
K HaradaA D Frankel
Current Opinion in Structural Biology
A D Frankel
© 2022 Meta ULC. All rights reserved