A novel group of oleosins is present inside the pollen of Arabidopsis

The Journal of Biological Chemistry
Hyun Uk KimAnthony H C Huang

Abstract

In plants, subcellular triacylglycerol granules in seeds (oil bodies) and floral tapetum (tapetosomes) are stabilized by amphipathic structural protein called oleosin. We hereby report a novel group of oleosins that is present inside the pollen of Arabidopsis thaliana. We have used the conserved sequence of oleosins to locate, via the DNA database, all 16 oleosin genes in the Arabidopsis genome. The oleosin genes can be divided into three groups according to their sequences and tissue-specific expressions, as probed by RNA blot hybridization and reverse transcriptase-PCR. The first group includes eight genes specifically expressed in the floret tapetum. The second group includes five genes specifically expressed in maturing seeds. The third, novel group includes three genes expressed in both maturing seeds and floral microspores, which will become pollen. Transgenic study using the promoter of one of these genes attached to a reporter gene has provided corroborative evidence for the specific expression of the gene in the microspores in the florets. One of the pollen oleosins can be identified by microsequencing and specific immunoblotting. Pollen oleosins synthesized by recombinant bacteria can collaborate with phospholipids in...Continue Reading

References

Apr 1, 1992·The Journal of Cell Biology·J T Tzen, A H Huang
Apr 1, 1992·Plant Molecular Biology·G J van RooijenM M Moloney
Aug 1, 1991·Plant Molecular Biology·R ScottJ Draper
May 1, 1990·The Plant Cell·D E de OliveiraJ Botterman
Dec 1, 1994·The Plant Journal : for Cell and Molecular Biology·L S RobertJ Simmonds
Dec 1, 1994·Plant Molecular Biology·K Lee, A H Huang
Apr 1, 1993·The Plant Journal : for Cell and Molecular Biology·D E de OliveiraM Van Montagu
Feb 7, 1997·The Journal of Biological Chemistry·J T TingA H Huang
Mar 1, 1997·The Plant Journal : for Cell and Molecular Biology·T W WangA H Huang
Oct 27, 1997·The Plant Cell·R K RuiterG J Wullems
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Apr 27, 1999·Trends in Biochemical Sciences·D J Murphy, J Vance
Jun 4, 1999·Seminars in Cell & Developmental Biology·C LondosA R Kimmel
Dec 22, 1999·Biochimica Et Biophysica Acta·V Narayanaswami, R O Ryan
May 20, 2000·Journal of Mammary Gland Biology and Neoplasia·I H Mather, T W Keenan
Oct 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S S WuA H Huang
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Jun 30, 2001·Science·J A MayfieldD Preuss
Jan 1, 1993·Plant Physiology·JTC. TzenAHC. Huang

❮ Previous
Next ❯

Citations

Jan 1, 2006·The Arabidopsis Book·Steven PenfieldIan A Graham
Jul 2, 2003·Current Opinion in Microbiology·Alexander Steinbüchel
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Sébastien BaudChristine Rochat
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chien Yu HuangAnthony H C Huang
Dec 1, 2011·The Journal of Biological Chemistry·Velayoudame ParthibaneRam Rajasekharan
May 4, 2013·Critical Reviews in Food Science and Nutrition·Jorge SáizCarmen García-Ruiz
May 1, 2007·Plant & Cell Physiology·Pei-Luen JiangJason T C Tzen
Jul 12, 2011·Plant & Cell Physiology·Ming-Der HuangAnthony H C Huang
Feb 21, 2004·Plant Physiology·Hyun Uk Kim, Anthony H C Huang
Nov 16, 2004·Plant Physiology·Kai Hsieh, Anthony H C Huang
May 1, 2007·Plant Physiology·Steven FootittFrederica L Theodoulou
Feb 9, 2013·Plant Physiology·Nan-Lan HuangAnthony H C Huang
Mar 2, 2010·Biological & Pharmaceutical Bulletin·Takashi L Shimada, Ikuko Hara-Nishimura
Mar 23, 2011·PLoS Genetics·Daniel BouyerArp Schnittger
Aug 12, 2014·PloS One·Yuan FangBrent D Mishler
Mar 19, 2013·The Arabidopsis Book·Yonghua Li-BeissonJohn Ohlrogge
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hironaka TsukagoshiKenzo Nakamura
Feb 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Aretha FiebigDaphne Preuss
Jun 24, 2014·Plant Physiology and Biochemistry : PPB·Buntora PasaribuJason T C Tzen
Feb 12, 2014·Plant Physiology·Martine MiquelBertrand Dubreucq
Aug 26, 2015·The New Phytologist·Madeleine Lévesque-LemayLaurian S Robert
Apr 23, 2013·Plant Science : an International Journal of Experimental Plant Biology·Toshiya SuzukiSumie Ishiguro
Jan 22, 2011·Plant Physiology and Biochemistry : PPB·Siam PopluechaiAjay Kohli
Oct 18, 2008·Comptes rendus biologies·Zita PurkrtovaThierry Chardot
Jun 14, 2015·Functional & Integrative Genomics·Mingli YuXinzheng Li
Aug 19, 2009·Journal of Integrative Plant Biology·Yu-Qing He, Yan Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.