A novel heme a insertion factor gene cotranscribes with the Thermus thermophilus cytochrome ba3 oxidase locus.

Journal of Bacteriology
Carolin WernerBernd Ludwig

Abstract

Studying the biogenesis of the Thermus thermophilus cytochrome ba(3) oxidase, we analyze heme a cofactor insertion into this membrane protein complex. Only three proteins linked to oxidase maturation have been described for this extreme thermophile, and in particular, no evidence for a canonical Surf1 homologue, required for heme a insertion, is available from genome sequence data. Here, we characterize the product of an open reading frame, cbaX, in the operon encoding subunits of the ba(3)-type cytochrome c oxidase. CbaX shares no sequence identity with any known oxidase biogenesis factor, and CbaX homologues are found only in the Thermaceae group. In a series of cbaX deletion and complementation experiments, we demonstrate that the resulting ba(3) oxidase complexes, affinity purified via an internally inserted His tag located in subunit I, are severely affected in their enzymatic activities and heme compositions in both the low- and high-spin sites. Thus, CbaX displays typical features of a generic Surf1 factor essential for binding and positioning the heme a moiety for correct assembly into the protein scaffold of oxidase subunit I.

References

Jun 1, 1987·Microbiological Reviews·C R Woese
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B H ZimmermannE Münck
May 30, 1997·The Journal of Biological Chemistry·G MashkevichA Tzagoloff
May 4, 2001·Biochimica Et Biophysica Acta·M M PereiraM Teixeira
May 21, 2003·Accounts of Chemical Research·Heather S Carr, Dennis R Winge
Apr 6, 2004·Nature Biotechnology·Anke HenneHans-Joachim Fritz
Jan 11, 2005·The Journal of Biological Chemistry·Stan J J BrounsJohn van der Oost
Mar 15, 2005·The Journal of Biological Chemistry·Daniel SmithJonathan P Hosler
Mar 16, 2005·Protein Expression and Purification·Ying ChenJames A Fee
Oct 4, 2005·Mitochondrion·Oleh Khalimonchuk, Gerhard Rödel
Apr 25, 2006·Biochimica Et Biophysica Acta·Paul A CobineDennis R Winge
Jun 9, 2006·American Journal of Physiology. Cell Physiology·Flavia FontanesiAntoni Barrientos
Aug 16, 2006·Biochimica Et Biophysica Acta·Daniela MooserBernd Ludwig
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jennifer M Zee, D Moira Glerum
Nov 8, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ivano Bertini, Gabriele Cavallaro
Jun 28, 2008·Biochimica Et Biophysica Acta·Freya A BundschuhBernd Ludwig
Sep 2, 2008·Nature Chemical Biology·Luciano A AbriataShenlin Wang
Jul 25, 2009·The Journal of Biological Chemistry·Freya A BundschuhBernd Ludwig

❮ Previous
Next ❯

Citations

Mar 6, 2012·Biochimica Et Biophysica Acta·Mohamed Radzi Noor, Tewfik Soulimane
May 5, 2012·Biochimica Et Biophysica Acta·Hyung J KimDennis R Winge
Nov 15, 2011·Biochimica Et Biophysica Acta·Seda EkiciFevzi Daldal
Sep 29, 2011·Biochimica Et Biophysica Acta·Achim HannappelBernd Ludwig
Jan 24, 2019·Metallomics : Integrated Biometal Science·Suraj Kumar MandalShankar Prasad Kanaujia
Aug 8, 2020·Genome Biology and Evolution·Mayank MahajanSiv G E Andersson
Sep 6, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Mauro Degli Esposti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.