PMID: 18187926Jan 12, 2008Paper

A novel high resolution in vivo digital imaging system for the evaluation of experimental cataract in diabetic rats

Journal of Pharmacological Sciences
Sokichi KametakaKunio Ishii

Abstract

The purpose of this study was to establish a novel imaging system for evaluation of cataract in small animals. Diabetic cataract was induced in male Wistar rats by a combination of a bolus injection of streptozotocin (65 mg/kg, i.v.) and 5% D-glucose given as drinking water. To assess cataract development, we designed a digital camera system equipped with a non-reflecting illuminator for capturing clear high-resolution full lens images in the horizontal plane. Cataract was evaluated from the resulting images using both an observer-based scoring system and quantitative digital image-analysis techniques. The onset of cataract was detected in the peripheral section of the lens in 57% of cases 2 weeks after induction of hyperglycemia. Central opacities were visible following 3 weeks in hyperglycemic conditions. The cataract increased in severity with time, so that by week 9 in hyperglycemic conditions, mature cataracts were developed in over 90% of lenses. Treatment of diabetic rats with GP-1447, an aldose reductase inhibitor, completely prevented the formation of diabetic cataracts. These results indicate that the digital imaging system established in the present study permits an assessment of all stages of cataract development an...Continue Reading

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Citations

Jun 13, 2012·Biological & Pharmaceutical Bulletin·Ken KawakuboKunio Ishii
Apr 24, 2008·Journal of Pharmacological Sciences·Sokichi KametakaKunio Ishii
Oct 28, 2019·Acta Ophthalmologica·Maria Della Volpe WaizelMargarita G Todorova
Oct 3, 2018·Biological & Pharmaceutical Bulletin·Yoshihiro HigashiTsutomu Nakahara

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