A novel, highly sensitive ALK antibody 1A4 facilitates effective screening for ALK rearrangements in lung adenocarcinomas by standard immunohistochemistry

Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer
Kim GruberClaudia Kalla

Abstract

Successful treatment of lung cancer patients with crizotinib depends on the accurate diagnosis of anaplastic lymphoma receptor tyrosine kinase (ALK) gene rearrangements. The approved fluorescence in-situ hybridization test is complex and difficult to use in daily diagnostic practice. Immunohistochemical assays-rapid and perfectly adapted for routine pathology practice-have been proposed as alternatives. We evaluated the novel high affinity ALK 1A4 antibody for routine diagnostics in formalin fixed, paraffin-embedded tumor specimens. Detection of ALK protein expression was investigated by comparing the new 1A4 antibody and the established D5F3 antibody/Ventana system in 218 lung cancer specimens with known ALK status preanalyzed by quantitative reverse transcription-polymerase chain reaction and fluorescence in-situ hybridization (20 ALK-positive cases, 198 ALK-negative cases). The accuracy of both immunohistochemical assays for the detection of ALK rearrangements was high. Using a conventional staining procedure without signal enhancement, the 1A4 antibody assay identified all 20 ALK-rearranged tumors (100% sensitivity) and correctly characterized 196 of 198 negative cases (99.1% specificity). The D5F3/Ventana assay detected 19...Continue Reading

References

Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Manabu SodaHiroyuki Mano
Feb 25, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mari Mino-KenudsonScott J Rodig
Oct 29, 2010·The New England Journal of Medicine·Eunice L KwakA John Iafrate
Jan 25, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Jin Ho PaikJin-Haeng Chung
Nov 11, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Anne McLeer-FlorinSylvie Lantuejoul
Jul 25, 2012·Virchows Archiv : an International Journal of Pathology·Erik ThunnissenGiulio Rossi
Nov 17, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Jong-Mu SunKeunchil Park
Dec 1, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Chris M J ConklinDiana N Ionescu
Jun 8, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Christina I SelingerBenjamin Solomon
Feb 6, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Kim GruberClaudia Kalla
May 3, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·John Le QuesneAndrew G Nicholson

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Citations

Apr 5, 2016·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Qiushi WangHualiang Xiao
Oct 20, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Qin ShenXiaojun Zhou
Jun 5, 2016·Surgical Oncology Clinics of North America·Min Zheng
Sep 13, 2016·Expert Review of Respiratory Medicine·Giuseppe PelosiAngelica Sonzogni
Jul 25, 2015·PloS One·Ilenia ChatziandreouAngelica A Saetta
Aug 31, 2016·Annals of Oncology : Official Journal of the European Society for Medical Oncology·C HeydtR Büttner
Dec 24, 2018·Expert Review of Anticancer Therapy·Yuka KozumaTakashi Seto
Jul 8, 2017·Archives of Pathology & Laboratory Medicine·Erik ThunnissenYasushi Yatabe
Oct 30, 2016·The International Journal of Biological Markers·Jung-Soo PyoJin Hee Sohn
Apr 21, 2021·Journal of Clinical Pathology·Esther CondeEnrique de Álava
Jun 27, 2021·Virchows Archiv : an International Journal of Pathology·Philipp JurmeisterMaximilian von Laffert

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