A novel histidine-tryptophan-ketoglutarate formulation ameliorates intestinal injury in a cold storage and ex vivo warm oxygenated reperfusion model in rats.

Bioscience Reports
Zhiquan ChenFelix Becker

Abstract

The present study aims to evaluate protective effects of a novel histidine-tryptophan-ketoglutarate solution (HTK-N) and to investigate positive impacts of an additional luminal preservation route in cold storage-induced injury on rat small bowels. Male Lewis rats were utilized as donors of small bowel grafts. Vascular or vascular plus luminal preservation were conducted with HTK or HTK-N and grafts were stored at 4°C for 8 h followed by ex vivo warm oxygenated reperfusion with Krebs-Henseleit buffer for 30 min. Afterwards, intestinal tissue and portal vein effluent samples were collected for evaluation of morphological alterations, mucosal permeability and graft vitality. The novel HTK-N decreased ultrastructural alterations but otherwise presented limited effect on protecting small bowel from ischemia-reperfusion injury in vascular route. However, the additional luminal preservation led to positive impacts on the integrity of intestinal mucosa and vitality of goblet cells. In addition, vascular plus luminal preservation route with HTK significantly protected the intestinal tissue from edema. HTK-N protected the intestinal mucosal structure and graft vitality as a luminal preservation solution. Additional luminal preservation ...Continue Reading

References

Jan 1, 1981·Laboratory Animals·C Moolenbeek, E J Ruitenberg
Jul 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R CariniE Albano
Jan 1, 1997·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·T MinorW Isselhard
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·U RauenH de Groot
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·J M WeinbergI Nissim
Jul 5, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Yasuhiro FujimotoThomas A Churchill
Jul 10, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·D OlsonT Churchill
Sep 16, 2003·Transplantation·David W OlsonThomas A Churchill
Sep 26, 2003·Transplantation·Payam SalehiThomas A Churchill
Aug 17, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Toshiaki TsujimuraThomas A Churchill
Nov 24, 2004·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Ursula Rauen, Herbert de Groot
Dec 17, 2004·Pediatric Surgery International·Igor SukhotnikEitan Shiloni
Apr 6, 2005·Transplantation Proceedings·H G D LeuveninkH van Goor
Dec 21, 2006·Cellular and Molecular Life Sciences : CMLS·U RauenH de Groot
Nov 21, 2007·Cryobiology·Ursula Rauen, Herbert de Groot
Mar 4, 2008·The Journal of Surgical Research·Ralf BahdeHans-Ullrich Spiegel
Jun 23, 2009·The Journal of Surgical Research·Judith StegemannThomas Minor
Sep 4, 2009·Cardiovascular Research·Christian JungJohn Pernow
Sep 4, 2009·The New England Journal of Medicine·Thomas M Fishbein
Oct 22, 2009·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Shengli WuUrsula Rauen
Oct 27, 2009·Nature Reviews. Immunology·Jerrold R Turner
Nov 17, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ingmar LautenschlägerStefan Uhlig
Dec 1, 2009·The Journal of Thoracic and Cardiovascular Surgery·Sivakkanan LoganathanGábor Szabó
Apr 28, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A S KleinA O Ojo
Nov 19, 2010·Transplant International : Official Journal of the European Society for Organ Transplantation·Anne Margot C RoskottRutger J Ploeg
Oct 15, 2011·Science·Malin E V Johansson, Gunnar C Hansson
Jan 10, 2012·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Nikolaus PizanisMarkus Kamler
Sep 22, 2012·Transplant International : Official Journal of the European Society for Organ Transplantation·Carl-Ludwig Fischer-FröhlichSilvio Nadalin
Dec 18, 2013·International Reviews of Immunology·Mihai Oltean, Thomas A Churchill
Feb 13, 2014·Immunological Reviews·Daniel N MoriDaniel R Goldstein
Feb 21, 2014·Current Opinion in Organ Transplantation·Ronald F Parsons, James V Guarrera
Nov 22, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Liara M GonzalezAnthony T Blikslager

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Citations

Sep 10, 2020·International Journal of Molecular Sciences·Annika MohrFelix Becker
Jan 5, 2021·Current Opinion in Organ Transplantation·Mihai Oltean

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