A novel host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei industrial strain RUT-C30

Biotechnology Letters
Lei ZhangDongzhi Wei

Abstract

To develop a host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei (teleomorph Hypocrea jecorina) industrial strain RUT-C30. The co-expression and purification system is based on: (i) an efficient and reliable selectable marker using bar (phosphinothricin acetyltransferase gene); (ii) a compact hygromycin B resistance marker; and (iii) a versatile integration plasmid for gene expression and knockout. Fluorescent protein genes were introduced into T. reesei and the corresponding proteins were purified from fermentation broth. The host-vector system was used in a proof-of-principle approach to achieve the co-expression of an alkaline endoglucanase and an alkaline cellobiohydrolase. This protocol can be used at an industrial scale to produce large amounts of proteins in T. reesei.

References

May 16, 2008·Fungal Genetics and Biology : FG & B·Manmeet Ahuja, Narayan S Punekar
Jan 17, 2009·Applied Microbiology and Biotechnology·Zinnia RahmanYasushi Morikawa
Sep 3, 2009·Biotechnology for Biofuels·Christian P KubicekBernhard Seiboth
Oct 15, 2011·Microbiology·Robyn Peterson, Helena Nevalainen
Sep 12, 2014·Journal of Industrial Microbiology & Biotechnology·Wei WangDongzhi Wei

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Citations

Jul 11, 2016·Trends in Biotechnology·Vijai Kumar GuptaRoberto N Silva
Jun 25, 2019·The Journal of General and Applied Microbiology·Chuan WuYushu Ma
Aug 23, 2019·Biotechnology Advances·Luísa Czamanski NoraRafael Silva-Rocha

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