A novel HPLC method for quantification of 10 antiepileptic drugs or metabolites in serum/plasma using a monolithic column

Therapeutic Drug Monitoring
Courtney HeideloffSihe Wang

Abstract

Therapeutic drug monitoring of antiepileptic drugs (AEDs) is important in maximizing the therapeutic response while minimizing the adverse effects. High-performance liquid chromatography (HPLC) is the most commonly used technique for this purpose. Recently, commercial monolithic columns were introduced, which consist of a single rod of fused silica or polymer. The objective of this work was to develop a simple and fast method to quantify 10 commonly measured AEDs or metabolites [carbamazepine, carbamazepine-10,11-epoxide, felbamate, lamotrigine, 10,11-dihydro-10-hydroxy-carbamazepine (active metabolite of oxcarbazepine), pentobarbital, phenobarbital, phenytoin, primidone, and zonisamide] in serum/plasma by HPLC using a reverse-phase monolithic column. Serum/heparin plasma (100 microL) was mixed with an internal standard solution (5-ethyl-5-p-tolylbarbituric acid in methanol, 250 microL). After centrifugation at 15,500 g for 10 minutes, 15 microL of supernatant was injected into a monolithic column. The analytes were eluted with an isocratic solution of 0.1 M, pH 6.5, phosphate buffer:methanol:acetonitrile (77:20:3), monitored at 210 nm. The chromatography time was 16 minutes. The method was linear from 0.4-4.9 to 21.2-190.9 mic...Continue Reading

References

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Citations

Feb 5, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Oscar NúñezRosa Busquets
Nov 1, 2012·Thérapie·Danièle Bentué-FerrerOlivier Tribut
Dec 20, 2015·Journal of Pharmaceutical and Biomedical Analysis·Fernando CarreñoTeresa Dalla Costa
May 25, 2011·Journal of Separation Science·Dustin R Bunch, Sihe Wang
Jun 11, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Juseop KangMin-Young Jun
Nov 14, 2014·Critical Reviews in Analytical Chemistry·Ana María Jiménez MorenoAgustín G Asuero
May 16, 2019·Microorganisms·Ricardo Franco-DuarteCélia F Rodrigues
Aug 28, 2019·Journal of Forensic Sciences·Thomai MouskeftaraNikolaos Raikos
Sep 8, 2018·Journal of Analytical Toxicology·Joana CarvalhoEugenia Gallardo
Feb 23, 2020·Scientific Reports·Yi-Kuang Yen, Chao-Yuan Chiu
Mar 7, 2021·Critical Reviews in Analytical Chemistry·Sajeeda SRuchi Verma
Jul 9, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kwabena F M OpuniCharles A Okai

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