A novel HSP70 gene of Schizosaccharomyces pombe that confers K-252a resistance

Gene
T UsuiS Horinouchi

Abstract

A new gene encoding a heat shock protein 70 family protein of Schizosaccharomyces pombe (Sp), named sks2+, was cloned as a weak suppressor for the K-252a-sensitive mutation, ucm1. The nucleotide sequence of sks2+ revealed an open reading frame of a 613-amino-acid (aa) protein. The deduced aa sequence of sks2+ showed significant homology with Saccharomyces cerevisiae (Sc) Ssb1p and Ssb2p responsible for protein synthesis by non-organelle-localized ribosomes, as well as with other proteins of the HSP70 family. The cells lacking the functional sks2+ gene were viable and showed no increased sensitivity to K-252a but grew slowly with an elongated morphology. These results suggest that the sks2+ gene product plays a role in the cell cycle progression and is able to confer drug resistance in a multicopy state.

References

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Citations

Oct 3, 2003·Biochimica Et Biophysica Acta·Bostjan CernilaKatja Breskvar
Mar 26, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takuhiro MatsumuraMitsuhiro Yanagida
Apr 30, 2016·Molecular & Cellular Proteomics : MCP·Thibaut LégerJean-Michel Camadro

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