A novel Hsp70 inhibitor prevents cell intoxication with the actin ADP-ribosylating Clostridium perfringens iota toxin

Scientific Reports
Katharina ErnstCordelia Schiene-Fischer

Abstract

Hsp70 family proteins are folding helper proteins involved in a wide variety of cellular pathways. Members of this family interact with key factors in signal transduction, transcription, cell-cycle control, and stress response. Here, we developed the first Hsp70 low molecular weight inhibitor specifically targeting the peptide binding site of human Hsp70. After demonstrating that the inhibitor modulates the Hsp70 function in the cell, we used the inhibitor to show for the first time that the stress-inducible chaperone Hsp70 functions as molecular component for entry of a bacterial protein toxin into mammalian cells. Pharmacological inhibition of Hsp70 protected cells from intoxication with the binary actin ADP-ribosylating iota toxin from Clostridium perfringens, the prototype of a family of enterotoxins from pathogenic Clostridia and inhibited translocation of its enzyme component across cell membranes into the cytosol. This finding offers a starting point for novel therapeutic strategies against certain bacterial toxins.

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Citations

Mar 4, 2020·Cells·Zarema AlbakovaAlexander M Sapozhnikov
Jul 13, 2016·Cellular Microbiology·Domenico Azarnia TehranCesare Montecucco
Mar 4, 2020·Nature Structural & Molecular Biology·Tomohito YamadaHideaki Tsuge
Apr 6, 2017·Scientific Reports·Manuel SchusterHolger Barth
Apr 11, 2018·Pathogens and Disease·Stephan FischerHolger Barth
Apr 6, 2021·Frontiers in Immunology·Zarema AlbakovaAlexander Sapozhnikov
May 20, 2021·Journal of Medicinal Chemistry·Andrew J Ambrose, Eli Chapman

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Methods Mentioned

BETA
protein folding
phase
dot blot
NMR
peptide array
flow cytometry
FCS

Software Mentioned

SPSS Sigmaplot
MicroCal
Origin

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