A novel human polycomb binding site acts as a functional polycomb response element in Drosophila.

PloS One
Suresh CuddapahXin Chen

Abstract

Polycomb group (PcG) proteins are key chromatin regulators implicated in multiple processes including embryonic development, tissue homeostasis, genomic imprinting, X-chromosome inactivation, and germ cell differentiation. The PcG proteins recognize target genomic loci through cis DNA sequences known as Polycomb Response Elements (PREs), which are well characterized in Drosophila. However, mammalian PREs have been elusive until two groups reported putative mammalian PREs recently. Consistent with the existence of mammalian PREs, here we report the identification and characterization of a potential PRE from human T cells. The putative human PRE has enriched binding of PcG proteins, and such binding is dependent on a key PcG component SUZ12. We demonstrate that the putative human PRE carries both genetic and molecular features of Drosophila PRE in transgenic flies, implying that not only the trans PcG proteins but also certain features of the cis PREs are conserved between mammals and Drosophila.

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Citations

Oct 1, 2013·Nature Structural & Molecular Biology·Chen DavidovichThomas R Cech
Nov 29, 2013·Nucleic Acids Research·Arindam BasuMichael L Atchison
Jan 21, 2015·Molecular Cell·Chen DavidovichThomas R Cech
Jul 6, 2014·Nucleic Acids Research·Chen DavidovichThomas R Cech
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Jun 9, 2015·Oncogene·M Koppens, M van Lohuizen
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Jun 14, 2017·Nucleic Acids Research·Stefan J Tekel, Karmella A Haynes
Jun 19, 2013·Molecular and Cellular Biology·Caroline J WooRobert E Kingston
Jan 5, 2019·RNA Biology·Junli YanWee-Joo Chng
Jun 15, 2017·Médecine sciences : M/S·Myriam KoubiEstelle Duprez
Sep 19, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hikaru Eto, Yusuke Kishi

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Methods Mentioned

BETA
ChIP-PCR
immunoprecipitation
ChIP
PCR
transgenic

Software Mentioned

Phospho Imager

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