A novel hydrogen peroxide sensor based on the direct electron transfer of horseradish peroxidase immobilized on silica-hydroxyapatite hybrid film

Biosensors & Bioelectronics
Bin WangHuai-Sheng Wang

Abstract

The direct electron transfer of immobilized horseradish peroxidase (HRP) on silica-hydroxyapatite (HAp) hybrid film-modified glassy carbon electrode (GCE) and its application as H(2)O(2) biosensors were investigated. On silica/HRP-HAp/GCE, HRP displayed a fast electron transfer process accompanied with one proton participate in. This sensor exhibited an excellent electrocatalytic response to the reduction of H(2)O(2) without the aid of an electron mediator. The proposed biosensor showed good reproducibility and high sensitivity to H(2)O(2) with the detection limit of 0.35 microM. In the range of 1.0-100 microM, the catalytic reduction current of H(2)O(2) was proportional to H(2)O(2) concentration. The apparent Michaelis-Menten constant (k(m)(app)) of the biosensor was calculated to be 21.8 microM, exhibiting a high enzymatic activity and affinity for H(2)O(2).

Citations

Jan 15, 2014·Biosensors & Bioelectronics·Jiachao YuSongqin Liu
Aug 12, 2015·Journal of Functional Biomaterials·Sergey V Dorozhkin
Jan 1, 2016·Biochemistry·Luciana HannibalRafael Radi
Dec 1, 2009·Analytical Biochemistry·Suiping WangRuqin Yu
Dec 17, 2015·Chemistry : a European Journal·Yun QiaoJun-ichi Kikuchi
Feb 19, 2011·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V V ShumiantsevaA I Archakov
Jun 15, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tatsuo NodaToshio Yao

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