A novel IgE-binding epitope of cat major allergen, Fel d 1

Biochemical and Biophysical Research Communications
Natt TasaniyanandaNitat Sookrung

Abstract

Information on the antigenic repertoire, especially the IgE-binding epitopes of an allergen is important for understanding the allergen induced immune response and cross-reactivity, as well as for generating the hypoallergenic variants for specific component resolved immunotherapy/diagnosis (CRIT and CRD). Data on the IgE-binding epitopes of cat allergens are scarce. In this study, a novel IgE-binding epitope of the cat major allergen, Fel d 1, was identified. Mouse monoclonal antibody (MAb) specific to the Fel d 1 was produced. Computerized intermolecular docking was used for determining the residues of the Fel d 1 bound by the specific MAb. The presumptive surface exposed residues of the Fel d 1 intrigued by the MAb are located on the chain 1. They are: L34 and T37 (helix 1); T39 (between helices 1 and 2); P40, E42 and E45 (helix 2); R61, K64, N65 and D68 (helix 3); and E73 and K76 (helix 4). The MAb competed efficiently with the cat allergic patients' serum IgE for Fel d 1 binding in the competitive IgE binding assay, indicating allergenicity of the MAb epitope. The newly identified allergenic epitope of the Fel d 1 is useful in a design of the CRIT and CRD for cat allergy.

References

Apr 1, 1991·Molecular Immunology·O A DuffortM Lombardero
Oct 1, 1990·The Journal of Allergy and Clinical Immunology·A J DabrowskiD Vervloet
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S SheriffD R Davies
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E A PadlanD R Davies
Jan 1, 1994·The Journal of Allergy and Clinical Immunology·F J van MilligenR C Aalberse
Jan 17, 1997·The Journal of Biological Chemistry·W van't HofA V Amerongen
Jun 26, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R ValentaH Grönlund
Dec 10, 1999·The Journal of Allergy and Clinical Immunology·R van ReeR C Aalberse
Feb 5, 2002·Journal of Clinical Microbiology·Patcharin SaengjarukHideo Hayashi
Mar 15, 2002·Current Allergy and Asthma Reports·W Phipatanakul
Jul 18, 2002·International Archives of Allergy and Immunology·Fátima FerreiraChristof Ebner
Jul 10, 2003·The Journal of Biological Chemistry·Liselotte KaiserGunter Schneider
Dec 21, 2004·Nucleic Acids Research·Ida RetterWerner Müller
May 17, 2006·Immunology and Allergy Clinics of North America·Mark Larché
Sep 1, 2009·Journal of Immunological Methods·Thomas TillerHedda Wardemann
Nov 27, 2009·International Archives of Allergy and Immunology·Anna Pomés
Feb 11, 2010·International Archives of Allergy and Immunology·Cécile Bienboire-FrosiniCatherine Ronin
Sep 8, 2010·The Journal of Allergy and Clinical Immunology·Andrea VeredaHugh A Sampson
Aug 30, 2011·The Journal of Allergy and Clinical Immunology·Fabio Dall'AntoniaWalter Keller
Oct 12, 2012·Bioinformatics·Ryan BrenkeDima Kozakov
Oct 19, 2012·Current Allergy and Asthma Reports·Regina Treudler, Jan C Simon
Jul 31, 2013·Methods : a Companion to Methods in Enzymology·Fabio Dall'antoniaWalter Keller
Sep 3, 2013·Proteins·Dima KozakovSandor Vajda

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Antibody Engineering

Antibody engineering technologies are constantly advancing to improve the clinical effectiveness of monoclonal and bispecific antibodies. Discover the latest research on Antibody Engineering here.

Antibody Repertoire Diversity

Antibody repertoire diversity and its role during natural infection is a prerequisite for molecular and structural elucidation of functionally protective immunity. Discover the latest insights into antibody diversity here.

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Related Papers

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
M CurinRudolf Valenta
The Journal of Allergy and Clinical Immunology
A Wesley BurksR M Helm
International Archives of Allergy and Immunology
Liselotte KaiserMarianne van Hage-Hamsten
The Journal of Allergy and Clinical Immunology
J L Ohman, F C Lowell
© 2022 Meta ULC. All rights reserved