A novel IgM class autoantibody to a hepatocyte-related 190 kDa molecule in patients with type 1 autoimmune hepatitis

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Katsumi YamauchiKeiko Shiratori

Abstract

It has been reported that autoantibodies to hepatocytes are frequently found in patients with autoimmune hepatitis (AIH). To elucidate the nature of these hepatocyte-specific autoantibodies, we attempted to generate a hepatocyte-specific monoclonal antibody (MoAb) from Epstein-Barr virus-transformed peripheral blood mononuclear cells obtained from a patient with AIH. We established a single clone, 2E3, that continued to produce an immunoglobulin M (IgM) antibody (lambda-type). This MoAb had the following properties: it reacted mainly with hepatocyte-derived cell lines, rather than with other cell lines, and it reacted with liver tissue but not with other tissues. By immunoblot analysis, we found that this MoAb recognized a 190 kDa molecule on hepatocytes. The MoAb was able to kill hepatocyte-derived cell lines in the presence of fresh human serum. This cytotoxic effect was completely abrogated by heat inactivation of human serum prior to its addition to cell lines. In addition, an IgM autoantibody that recognized a 190 kDa molecule was also found in patients with AIH but not in those with chronic hepatitis C; its titer correlated significantly with serum alanine aminotransferase (ALT) levels in patients with AIH. In conclusion,...Continue Reading

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Citations

Apr 20, 2007·Biochimica Et Biophysica Acta·Cecilia L BasiglioAldo D Mottino
May 27, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Katsumi YamauchiKeiko Shiratori
Jun 18, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Michael A Heneghan, Ian G McFarlane
Oct 8, 2009·Clinical Science·Cecilia L BasiglioAldo D Mottino

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Autoimmune hepatitis formerly called lupoid hepatitis, is a chronic, autoimmune disease of the liver that occurs when the body's immune system attacks liver cells causing the liver to be inflamed. Discover the latest research on autoimmune hepatitis here.

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