A novel immobilization strategy for electrochemical detection of cancer biomarkers: DNA-directed immobilization of aptamer sensors for sensitive detection of prostate specific antigens

The Analyst
Zhugen YangPedro Estrela

Abstract

We report on a novel strategy for DNA aptamer immobilization to develop sensitive electrochemical detection of a protein biomarker, with prostate specific antigen (PSA) as a case biomarker. Thiolated single-stranded DNA (ssDNA) was co-immobilized with 3-mercapto-1-propanol on gold electrodes, and used as a scaffold for DNA aptamer attachment through hybridization of the aptamer overhang (so-called "DNA-directed immobilization aptamer sensors", DDIAS). In the approach, the complementary DNA aptamer against PSA was assembled by the probe ssDNA onto the electrode to detect PSA; or the probe ssDNA directly hybridized with a complementary DNA aptamer/PSA complex following their pre-incubation in solution, so-called 'on-chip' and 'in-solution' methods, respectively. A double stranded DNA intercalator with a ferrocenyl (Fc) redox marker was synthesized to evaluate the feasibility of the strategy. The results demonstrate that the 'in-solution' method offers a favourable medium (in a homogeneous solution) for the binding between the aptamer and PSA, which shows to be more efficient than the 'on-chip' approach. DDIAS shows promising analytical performance under optimized conditions, with a limit of detection in the range of fM and low no...Continue Reading

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Citations

Jul 3, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongguang Sun, Youli Zu
Feb 13, 2016·The Analyst·Muslum Ilgu, Marit Nilsen-Hamilton
Dec 1, 2015·The Analyst·Hongguang SunYouli Zu
Mar 5, 2016·Cancer Investigation·Zhihao ZhangXiaoyan Zhao
Nov 10, 2013·PloS One·M Elena MartínVíctor M González
Jun 25, 2016·Nano Letters·Ioulia TzouvadakiSandro Carrara
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Oct 18, 2016·Bioconjugate Chemistry·Alejandra TelloHumberto Gomez
Oct 16, 2019·Electrophoresis·Worapot Suntornsuk, Leena Suntornsuk
Oct 18, 2016·Mass Spectrometry Reviews·Félix HernándezLubertus Bijlsma
Feb 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sayeh Dowlatshahi, Mohammad J Abdekhodaie
Aug 18, 2017·Analytical Chemistry·Zhugen YangJonathan M Cooper

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