A novel immortalization vector for the establishment of penaeid shrimp cell lines.

In Vitro Cellular & Developmental Biology. Animal
Guo-Bin HuKun-Feng Yang

Abstract

Cell immortalization technology based on gene transfer has been successfully used to generate cell lines from a wide variety of cell types. The inability to stably introduce and express foreign genes has hampered application of this strategy in shrimp cells. We report here the use of replication-defective pantropic retrovirus to achieve a novel immortalization vector in which simian virus 40 large T antigen (SV40T) gene is expressed from Moloney murine leukemia virus (MoMLV) promoter. Data confirmed the presence of transferred SV40T gene and its stable mRNA expression in transduced lymphoid cells of Penaeus chinensis. The transduced cells showed a higher growth rate and a longer replication life-span compared with their untransduced counterparts. These results indicate the pantropic retrovirus-based immortalization-inducing gene delivery system is a potential tool for establishing cell lines from shrimp.

References

Jul 31, 1989·Biochemical and Biophysical Research Communications·M Díaz-GuerraJ Renart
Apr 1, 1995·Veterinary Immunology and Immunopathology·J R Stabel, T J Stabel
Jan 1, 1994·Critical Reviews in Oncogenesis·T M Bryan, R R Reddel
May 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·L M TapayP C Loh
May 26, 1999·Molecular and Biochemical Parasitology·X QueS L Reed
Jun 8, 2001·Veterinary Immunology and Immunopathology·S KöksoyL E Mathes
Aug 21, 2001·Biochemical and Biophysical Research Communications·S MizuaraiS Iijima
Aug 23, 2001·In Vitro Cellular & Developmental Biology. Animal·C ShimizuJ C Burns
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jean-Etienne AllainAnne Weber
Mar 6, 2004·Pathologie-biologie·Anne Weber
Nov 17, 2004·Cell Stress & Chaperones·G I LancasterL Nybo
Sep 1, 2005·Marine Biotechnology·Baruch Rinkevich
Sep 29, 2006·Biochemical and Biophysical Research Communications·Shigehisa KawataTomoyuki Shishido

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Citations

Sep 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·P JayeshI S Bright Singh
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amanda B CoreCynthia A Bradham
Aug 19, 2015·Fish & Shellfish Immunology·Jayesh PuthumanaI S Bright Singh
Sep 15, 2016·Cell and Tissue Research·Claudette RabinowitzBaruch Rinkevich

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Methods Mentioned

BETA
transfection
PCR
transgenic

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