A novel impedance-based cellular assay for the detection of anti-calcium channel autoantibodies in type 1 diabetes

Journal of Immunological Methods
M W Jackson, Tom P Gordon

Abstract

We have recently postulated that functional autoantibodies (Abs) against L-type voltage-gated calcium channels (VGCCs) contribute to autonomic dysfunction in type 1 diabetes (T1D). Previous studies based on whole-organ assays have proven valuable in establishing the mechanism of anti-VGCC Ab activity, but are complex and unsuitable for screening large patient cohorts. In the current study, we used real-time dynamic monitoring of cell impedance to demonstrate that anti-VGCC Abs from patients with T1D inhibit the adherence of Rin A12 cells. The functional effect of the anti-VGCC Abs was mimicked by the dihydropyridine agonist, Bay K8644, and reversed by the antagonist, nicardipine, providing a pharmacological link to the whole-organ studies. IVIg neutralized the effect on cell adhesion of the anti-VGCC Abs, consistent with the presence of anti-idiotypic Abs in IVIg that may prevent the emergence of pathogenic Abs in healthy individuals. The cell impedance assay can be performed in a 96 well plate format, and represents a simple method for detecting the presence of anti-VGCC activity in patient immunoglobulin (IgG). The new cell assay should prove useful for further studies to determine the prevalence of the Ab and its association...Continue Reading

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Citations

Dec 24, 2011·Assay and Drug Development Technologies·Timo WeilandSascha Venturelli
Mar 17, 2006·Pathologie-biologie·N Fabien, J-C Monier
Aug 26, 2018·Translational Research : the Journal of Laboratory and Clinical Medicine·Lian YiWei-Jun Qian

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