A novel inhibition ELISA for the detection and monitoring of Penicillium marneffei antigen in human serum

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
K PrakitS Youngchim

Abstract

The thermally dimorphic fungus Penicillium marneffei is a causative agent of penicilliosis marneffei, a disease considered to be an acquired immune deficiency syndrome (AIDS)-defining illness in Southeast Asia and southern China. We have developed an inhibition enzyme-linked immunosorbent assay (inh-ELISA) incorporating the yeast phase specific mannoprotein-binding monoclonal antibody 4D1 for the detection of P. marneffei infection. In our sample set, the test detected antigenemia in all 45 (100 %) patients with P. marneffei, with a mean antigen concentration of 4.32 μg/ml. No cross-reactivity in this assay was found using serum from 44 additional patients with other fungal infections, such as Aspergillus fumigatus, Cryptococcus neoformans, and Candida albicans, as well as 44 patients with bacterial infections, such as Mycobacterium tuberculosis and Streptococcus suis. Additionally, no reactivity occurred using serum from 31 human immunodeficiency virus (HIV)-infected patients without a history of fungal infections and 113 healthy controls residing in endemic areas. To investigate the potential of the inh-ELISA for disease monitoring, we followed the reduction in antigenemia in six patients who clinically responded to itraconaz...Continue Reading

References

Aug 13, 1994·Lancet·K Y YuenP Y Chau
Oct 1, 1996·Journal of Clinical Microbiology·L KaufmanT D Mastro
Oct 8, 1997·Journal of Clinical Microbiology·B L GomezR J Hay
Oct 27, 1999·Journal of Medical Microbiology·S S WongK Y Yuen
Mar 4, 2000·International Journal of STD & AIDS·F J VilarN P Jones
Aug 31, 2002·Journal of Clinical Microbiology·Varunee DesakornNicholas J White
Jan 9, 2003·Journal of Clinical Microbiology·Sansanee C ChaiyarojYuttana Srinoulprasert
Aug 9, 2003·Journal of Clinical Microbiology·Silvia Helena Marques da SilvaZoilo Pires de Camargo
Jun 9, 2004·Journal of Clinical Microbiology·Silvia Helena Marques da SilvaZoilo Pires De Camargo
Oct 9, 2004·Journal of Clinical Microbiology·Silvia Helena Marques da SilvaZoilo Pires de Camargo
Jan 6, 2005·Microbiology·Sirida YoungchimAndrew J Hamilton
Jan 19, 2006·Clinical Microbiology Reviews·Nongnuch VanittanakomThira Sirisanthana
Mar 11, 2009·Medical Mycology·Nongnuch VanittanakomThira Sirisanthana
Sep 25, 2009·Clinical and Vaccine Immunology : CVI·Rodrigo BerzaghiZoilo Pires de Camargo

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Citations

Apr 22, 2017·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Juan PengQingping Jiang
Aug 24, 2017·Current Infectious Disease Reports·Daniel B ChastainCarlos Franco-Paredes
Dec 4, 2020·Open Forum Infectious Diseases·Vo Trieu LyThuy Le
Jun 22, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nguyen T M ThuThuy Le
Dec 15, 2019·Diagnostic Microbiology and Infectious Disease·Kritsada PruksaphonSirida Youngchim
Jun 3, 2021·Diagnostics·Victoria PoplinNathan C Bahr
Jul 16, 2021·Frontiers in Cellular and Infection Microbiology·Zheng-Tu LiFeng Ye

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