A novel inhibitor of 9-cis-epoxycarotenoid dioxygenase in abscisic acid biosynthesis in higher plants

Plant Physiology
Sun-Young HanTadao Asami

Abstract

Abscisic acid (ABA) is a major regulator in the adaptation of plants to environmental stresses, plant growth, and development. In higher plants, the ABA biosynthesis pathway involves the oxidative cleavage of 9-cis-epoxycarotenoids, which may be the key regulatory step in the pathway catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED). We developed a new inhibitor of ABA biosynthesis targeting NCED and named it abamine (ABA biosynthesis inhibitor with an amine moiety). Abamine is a competitive inhibitor of NCED, with a Ki of 38.8 microm. In 0.4 m mannitol solution, which mimics the effects of osmotic stress, abamine both inhibited stomatal closure in spinach (Spinacia oleracea) leaves, which was restored by coapplication of ABA, and increased luminescence intensity in transgenic Arabidopsis containing the RD29B promoter-luciferase fusion. The ABA content of plants in 0.4 m mannitol was increased approximately 16-fold as compared with that of controls, whereas 50 to 100 microm abamine inhibited about 50% of this ABA accumulation in both spinach leaves and Arabidopsis. Abamine-treated Arabidopsis was more sensitive to drought stress and showed a significant decrease in drought tolerance than untreated Arabidopsis. These results...Continue Reading

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Citations

Apr 5, 2011·Journal of Plant Research·Nobutaka Kitahata, Tadao Asami
Dec 23, 2008·The Journal of Biological Chemistry·Martin J SergeantAndrew J Thompson
Jun 5, 2010·Plant & Cell Physiology·Shinsaku ItoTadao Asami
Oct 12, 2012·Plant & Cell Physiology·Yoshiya SetoJunko Kyozuka
Jun 5, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Shukui GuoMichèle C Loewen
Nov 15, 2013·Molecular Plant-microbe Interactions : MPMI·Mazen AlazemNa-Sheng Lin
Jun 30, 2006·Plant Biology·A ChristmannE Grill
Jan 16, 2007·The Plant Journal : for Cell and Molecular Biology·Jaimie M Van Norman, Leslie E Sieburth
Sep 29, 2011·The Plant Journal : for Cell and Molecular Biology·John C GrayChristine A Newell
Jun 28, 2006·Yi chuan xue bao = Acta genetica Sinica·Jun YouLi-Zhong Xiong
Feb 2, 2010·Plant Signaling & Behavior·Akiyoshi TominagaAkihiro Suzuki
Oct 31, 2009·Plant Signaling & Behavior·María del Carmen Rodríguez-GacioAngel J Matilla
Nov 22, 2014·Frontiers in Plant Science·Hidemitsu Nakamura, Tadao Asami
May 11, 2005·Bioorganic & Medicinal Chemistry·Nobutaka KitahataTadao Asami
Apr 22, 2018·Bioscience, Biotechnology, and Biochemistry·Kai Jiang, Tadao Asami
Jun 23, 2018·Plant & Cell Physiology·Yuichiro Tsuchiya
Jul 10, 2018·Plant & Cell Physiology·Wim DejongheSean R Cutler
Oct 28, 2019·Plant Biotechnology Journal·Gianfranco DirettoGiovanni Giuliano
Mar 10, 2016·Journal of Plant Research·Ken-Ichi OsukiToshiki Uchiumi
Apr 5, 2017·Frontiers in Plant Science·Sajjad Z AwanAndrew J Thompson
Oct 23, 2013·Archives of Biochemistry and Biophysics·Peter J Harrison, Timothy D H Bugg

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