A novel insulin-releasing substance, phanoside, from the plant Gynostemma pentaphyllum.

The Journal of Biological Chemistry
Ake NorbergC G Ostenson

Abstract

Extracts from Gynostemma pentaphyllum Makino (Cucurbitaceae), a Southeast Asian herb, has been reported to affect numerous activities resulting in antitumor, cholesterol-lowering, immunopotentiating, antioxidant, and hypoglycemic effects. We have isolated one active compound by ethanol extraction, distribution in n-butyl alcohol/water, solid phase extraction/separation, and several rounds of reverse phase high pressure liquid chromatography. We have shown by NMR and mass spectrometry that this active compound is a novel saponin, a gypenoside, which we have named phanoside (21-,23-epoxy-,3beta-,20-,21-trihydroxydammar-24-ene-3-O-([alpha-d-rhamnopyranosyl(1-->2)]-[beta-d-glycopyranosyl(1-->3)]-beta-d-lyxopyranoside)), with a molecular mass of 914.5 Da. Phanoside is a dammarane-type saponin, and four stereoisomers differing in configurations at positions 21 and 23 were identified, each of which were found to stimulate insulin release from isolated rat pancreatic islets. We have also found that the stereoisomers are interconvertible. Dose-dependent insulin-releasing activities at 3.3 and 16.7 mM glucose levels were determined for the racemic mixture containing all four stereoisomers. Phanoside at 500 microM stimulates insulin relea...Continue Reading

References

Dec 15, 1992·European Journal of Pharmacology·G PanagiotidisI Lundquist
Aug 1, 1990·Diabetes Care·W J Malaisse, P Lebrun
Jun 1, 1989·Journal of Ethnopharmacology· Atta-Ur-Rahman, K Zaman
Oct 1, 1965·The Journal of Clinical Endocrinology and Metabolism·V HerbertS J Bleicher
May 1, 1995·Journal of Ethnopharmacology·B la CourZ Yi
Dec 1, 1996·Journal of Natural Products·L HuY Xie
Oct 27, 1999·Nitric Oxide : Biology and Chemistry·M A TannerP R Myers
Oct 18, 2001·Journal of the American College of Nutrition·V VuksanM P Stavro
Jun 6, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·J T XieC S Yuan
Oct 14, 2003·Drug Discovery Today·Marcus Anhäuser

❮ Previous
Next ❯

Citations

Mar 26, 2011·Plant Foods for Human Nutrition·Swapnil Balasaheb PatilAkalpita Umesh Arvindekar
Nov 24, 2012·Journal of Agricultural and Food Chemistry·Jing-Guang LuZhi-Hong Jiang
Jun 11, 2008·The American Journal of Chinese Medicine·Wen-Chung HuangJiann-Jong Shen
Oct 29, 2010·Evidence-based Complementary and Alternative Medicine : ECAM·B Shivananda NayakAndrew Adogwa
May 3, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Wen-Chung HuangJiann-Jong Shen
May 16, 2006·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Zhi-Xiong XiangYing Qin
Jun 12, 2013·Canadian Journal of Physiology and Pharmacology·Carani Venkatraman Anuradha
Jan 30, 2010·International Journal of Food Sciences and Nutrition·Sirichai AdisakwattanaTipayanate Ariyapitipan
Nov 5, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ko-Chung TsuiJyh-Feng Lu
Aug 7, 2012·Expert Opinion on Investigational Drugs·Tian-Tian Zhang, Jian-Guo Jiang
Apr 21, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·L SchildR Brödemann
Jun 19, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chian-Jiun LiouJiann-Jong Shen
May 21, 2010·Phytochemistry·Yongmei HuNancy Y Ip
Apr 13, 2010·Phytochemistry·Pham Thanh KyYoung Ho Kim
Jan 26, 2010·Phytochemistry·Zhen ZhangJin-Feng Hu
Apr 29, 2009·Journal of Ethnopharmacology·Tran Manh HungByung-Sun Min
Nov 6, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·V T T HuyenC G Ostenson
Feb 23, 2013·Journal of Nutrition and Metabolism·V T T HuyenC G Ostenson
May 29, 2012·Journal of Ethnopharmacology·K Hoa NguyenB L Grégoire Nyomba
Jul 23, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Ezarul Faradianna LokmanClaes-Göran Östenson
Jun 26, 2013·Journal of Ethnopharmacology·Natthakarn ChiranthanutNirush Lertprasertsuk
May 14, 2011·Phytochemistry·Ji Ho Kim, Yong Nam Han
Jan 29, 2016·PloS One·Verena StadlbauerJulian Weghuber
Jun 8, 2017·Nutrients·Aline Carla InadaRita de Cássia Avellaneda Guimarães
Jun 27, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sun-A ImChong Kil Lee
Aug 17, 2019·Current Vascular Pharmacology·Hoa Thi PhuGianfranco Pintus
Dec 19, 2017·Experimental and Therapeutic Medicine·Gerburg KeilhoffLorenz Schild
Oct 29, 2014·Archives of Pharmacal Research·Dao-Jin ChenXiang-Lan Piao
Mar 5, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Minxia PangGuiyuan Lv
Nov 14, 2020·Journal of Ethnopharmacology·Ngoc-Hieu NguyenWon Keun Oh
May 1, 2021·International Journal of Molecular Sciences·Adelina LombreaCorina Danciu
Jul 30, 2021·Journal of Human Nutrition and Dietetics : the Official Journal of the British Dietetic Association·Amanda RaoDavid Briskey

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.