A novel intragenic sequence enhances initiator-dependent transcription in human embryonic kidney 293 cells.

The Journal of Biological Chemistry
Chiara AbresciaPierpaolo Di Nocera

Abstract

In a variety of Drosophila TATA-less promoters, transcription is directed by initiator (Inr) sequences, which are faithfully and efficiently recognized only when flanked 3' by the downstream promoter element (DPE). This motif, which is conserved at approximately 30 bp from the RNA start site, is viewed as a downstream counterpart to the TATA box, and is recognized by the general transcription factor (TF) IID. By transient expression assays in human embryonic kidney 293 cells, we show that DE1 (distal element 1), a DNA motif located at residues +23 to +29, sustains faithful Inr-dependent transcription as efficiently as the DPE. Transcription significantly increased when DE1 and DPE sequences were adjacently placed on the same template. Results emerging from in vivo RNA analyses matched electrophoretic mobility shift assay data. In agarose-electrophoretic mobility shift assays, retarded DNA-protein complexes resulting from the interaction of human holo-TFIID with either Inr(+)/DPE(+) or Inr(+)/DE1(+) promoters were formed at comparable levels, whereas binding of TFIID to both DE1 and DPE motifs was 2-fold increased. The strict requirement for spacing between the Inr and DPE was not observed for DE1, as locating the motif 4 bp awa...Continue Reading

References

Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G Grimaldi, P P Di Nocera
Nov 1, 1995·Molecular and Cellular Biology·K H EmamiS T Smale
Jan 1, 1995·Annual Review of Biochemistry·L Zawel, D Reinberg
Nov 3, 1995·The Journal of Biological Chemistry·C ContursiP P Di Nocera
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Colgan, J L Manley
Dec 10, 1998·Nucleic Acids Research·R C PérierP Bucher
Jan 26, 1999·Journal of Molecular Biology·C LiberatiR Mantovani
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·B A LewisS H Orkin
Oct 3, 2001·Genes & Development·J E Butler, J T Kadonaga
Nov 2, 2001·The Journal of Biological Chemistry·Mattia FrontiniRoberto Mantovani

❮ Previous
Next ❯

Citations

Oct 28, 2004·Journal of Virology·Sandra PauliThomas Hohn
Aug 26, 2003·Journal of Cellular Biochemistry·Bo ShiRonald B Gartenhaus
Jan 13, 2006·Gene·Fabrizio BologneseRoberto Mantovani

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.