PMID: 7545308Apr 1, 1995Paper

A novel IRMA and ELISA for quantifying cytokeratin 8 and 18 fragments in the sera of healthy individuals and cancer patients

Scandinavian Journal of Clinical and Laboratory Investigation
A SilénS Nilsson

Abstract

Cytokeratins 8 and 18 are most frequently co-expressed in simple epithelia and carcinomas. Fragments of these cytokeratins are released into the systemic circulation where they can be quantified and utilized as tumour markers. The present paper reports on a solid-phase sandwich monoclonal immunoassay, considered "epithelial tissue-specific", which recognizes fragments of human cytokeratins 8 and 18 of different sizes (10-50 kD). The evaluated ELISA and IRMA assays exhibited a detection limit of 0.1 microgram 1-1 and characteristically demonstrated a within-assay coefficient of variation (CV) of 1-4% and a between-assay CV of 3-5%. The long-term (300 days) repeatability of lyophilized samples tested in the assay was estimated to have a less than 10% CV. Of apparently healthy individuals, 95% were found to have serum concentrations of less than 0.95 micrograms 1-1. A highly significant difference was found between apparently healthy individuals and pancreatic cancer patients. Patients with metastatic disease showed a sensitivity of 93% and those with local disease 83%, at 95% specificity. This TPAcyk assay revealed good correlation (0.98) with the TPS assay detecting the specific M3 epitope of the tissue polypeptide antigen. The ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1990·British Journal of Urology·A LewenhauptB Nilsson
Oct 15, 1990·International Journal of Cancer. Journal International Du Cancer·B Sundström, T Stigbrand
Jan 1, 1985·Annals of the New York Academy of Sciences·R A QuinlanW W Franke
Aug 11, 1983·Biochimica Et Biophysica Acta·B Geiger
Jul 24, 1980·Biochimica Et Biophysica Acta·B LüningB Björklund
Jan 1, 1980·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·P RedeliusB Björklund
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Jun 21, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Faruk TasDerya Duranyildiz
Dec 4, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Faruk TasDerya Duranyildiz
Jun 17, 1998·International Journal of Cancer. Journal International Du Cancer·B BrandtK S Zänker
Jul 3, 2013·Melanoma Research·Faruk TasDerya Duranyildiz

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