A novel lecithin:cholesterol acyltransferase for soybean oil refining provides higher yields and extra nutritional value with a cleaner process

Applied Microbiology and Biotechnology
Guillermo HailsHugo G Menzella

Abstract

The growing demand for food and biofuels urges the vegetable oil processing industry to adopt cleaner technologies to mitigate the environmental pollution caused by chemical refining processes. Over the past decade, several enzymatic methods have proven to be efficient at reducing the generated waste, but improving the benefit-cost ratio is still necessary for the widespread adoption of this technology. In this work, we show that lecithin:cholesterol acyltransferase from Aeromonas enteropelogenes (LCATAE) provides a higher extra-yield of soybean oil than a type A1 phospholipase (PLA) enzyme currently commercialized for soybean oil deep degumming. Our model indicates that crude soybean oil treated with the new enzyme generates 87% more neutral oil from phospholipids than the widely used PLA, with the corresponding reduction in waste and byproducts generation. The refined oil retains the phytosterols naturally present in crude oil, enriching its nutritional value. The results presented here position LCATAE as a promising candidate to provide the green solutions needed by the industrial oil processing sector. Key points • Selected LCAT gene candidates were expressed in E. coli. • Aeromonas enteropelogenes LCAT hydrolyzes all the p...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Apr 5, 2003·Bioresource Technology·G D Haki, S K Rakshit
Apr 28, 2004·Journal of Molecular Biology·Lukas KällErik L L Sonnhammer
Nov 4, 2004·Progress in Lipid Research·Casimir C AkohJei-Fu Shaw
Jun 15, 2010·Applied and Environmental Microbiology·Hugo G MenzellaDaniel V Santi
Jan 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Maria Cristina RulliPaolo D'Odorico
Nov 23, 2013·Applied Microbiology and Biotechnology·Andres AguirreHugo G Menzella
Feb 3, 2015·Current Protocols in Protein Science·Kathy Huynh, Carrie L Partch
Nov 1, 2015·Journal of Biotechnology·Pablo RavasiHugo G Menzella
Feb 27, 2017·Applied Microbiology and Biotechnology·Sebastián CerminatiHugo G Menzella
Nov 8, 2017·International Journal of Medical Microbiology : IJMM·Miriam HillerAntje Flieger
Jun 18, 2018·Applied Microbiology and Biotechnology·Sebastián CerminatiMaría Eugenia Castelli
Feb 8, 2019·Applied Microbiology and Biotechnology·Sebastián CerminatiMaría Eugenia Castelli

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Citations

May 1, 2021·International Journal of Molecular Sciences·Il-Sup KimWoong-Suk Yang

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Datasets Mentioned

BETA
MG774550
MG774551
MG774552
MG774553
MG774554
MG774555

Methods Mentioned

BETA
PCR
NMR
thermal shift
acylation

Software Mentioned

LCAT
Phobius
GenScript
LCAT AE
ExPASy
ProtParam
BLASTp

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