A novel lipopeptide produced by a Pacific Ocean deep-sea bacterium, Rhodococcus sp. TW53

Journal of Applied Microbiology
F PengZongze Shao

Abstract

Our goal was to find a novel, biosurfactant-producing bacterium from Pacific Ocean deep-sea sediments. An oil-degrading biosurfactant-producing bacterium TW53 was obtained from deep-sea sediment, and was identified through 16S rDNA analysis as belonging to the genus Rhodococcus. It lowered the surface tension of its culture to 34.4 mN m(-1). Thin layer chromatography (TLC) showed that the crude biosurfactants of TW53 were composed of lipopeptides and free fatty acids (FA). The lipopeptides were purified with column chromatography and then hydrolysed with 6 mol l(-1) HCl. Gas chromatography-mass spectrometry analysis showed that the hydrolyte in the hydrophobic fraction contained five kinds of FA with chain lengths of C(14)-C(19), and C(16)H(32)O(2) was a major component making up 59.18% of the total. However, 3-hydroxyl FA was not found, although it is usually found in lipopeptides. Silica gel TLC revealed that the hydrolyte in the hydrophilic fraction was composed of five kinds of amino acids; consistently, ESI-Q-TOF-MS analysis confirmed the composition results and provided their sequence tentatively as Ala-Ile-Asp-Met-Pro. Furthermore, the yield and CMC (critical micelle concentrations) of purified lipopeptides were examined...Continue Reading

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Citations

Nov 3, 2010·Bioprocess and Biosystems Engineering·C SivapathasekaranRamapati Samanta
Sep 13, 2011·AMB Express·Randhir S MakkarIbrahim M Banat
Jan 15, 2014·Extremophiles : Life Under Extreme Conditions·Susana Bequer UrbanoHéctor M Alvarez
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Aug 25, 2021·Chemosphere·Lucélia CabralLara Durães Sette
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