A novel lipophilic spin probe for the measurement of radiation damage in mouse brain using in vivo electron spin resonance (ESR)

FEBS Letters
Y MiuraToshihiko Ozawa

Abstract

As a possible lipophilic spin probe of in vivo electron spin resonance (ESR), 3-methoxy carbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-yloxy (MCPROXYL) was examined. The permeability of the blood-brain barrier to this compound was evaluated with a brain uptake index and autoradiography, with result that this probe is well distributed in the brain. The in vivo ESR spectra were measured in the head and the abdomen of MCPROXYL-injected living mice. The rate of signal decay of MCPROXYL in the head measured at one hour after X-irradiation was about 75% of that of the controls. The decrease in the head seems to be related to the early response of the brain to X-irradiation. This is the first report that the behavior of free radical such as MCPROXYL in the brain is influenced by X-irradiation. MCPROXYL is thus useful as a novel spin probe for in vivo ESR to monitor the radiation damage in the brain.

References

Feb 25, 1975·Biochemistry·S Hong-wei, H McConnell
Feb 14, 1992·Biochemical and Biophysical Research Communications·Y MiuraA Hamada
Jun 15, 1994·Biochemical and Biophysical Research Communications·A M KomarovC S Lai

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Citations

May 10, 2000·Free Radical Biology & Medicine·Y Miura, T Ozawa
Aug 11, 2004·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Masaichi-Chang-Il LeeToshihiko Ozawa
Oct 8, 2005·Magnetic Resonance in Medical Sciences : MRMS : an Official Journal of Japan Society of Magnetic Resonance in Medicine·Masaichi-Chang-Il LeeToshihiko Ozawa
Jan 24, 2013·Journal of Clinical Biochemistry and Nutrition·Masaichi-Chang-Il Lee
Feb 15, 2011·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Teruhito KunimatsuMasaichi-Chang-il Lee
Feb 14, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fuminori HyodoMurali C Krishna
Aug 14, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jiangang ShenKe Jian Liu
Apr 15, 2005·Redox Report : Communications in Free Radical Research·Shinjiro MiyakeMasaichi-Chang-il Lee
Apr 12, 2003·Redox Report : Communications in Free Radical Research·Hiroyuki MiyazakiMasaichi-Chang-il Lee
May 9, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jiangang ShenKe Jian Liu
Oct 13, 2012·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Hirotada Fujii, Miho Emoto
Dec 23, 2004·Journal of Radiation Research·Keizo Takeshita, Toshihiko Ozawa
Sep 29, 2015·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Ken-Ichiro MatsumotoKen-Ichi Yamada
Sep 10, 2020·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Keizo TakeshitaShoko Okazaki
Aug 26, 2019·Free Radical Biology & Medicine·Mizuki NakamuraKen-Ichiro Matsumoto
Jul 16, 2003·Archives of Biochemistry and Biophysics·Hideo Utsumi, Ken-ichi Yamada

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