A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis

Oncotarget
Mitsuhiro ShimuraMichiaki Unno

Abstract

The aim of this study was to identify novel liver metastasis-correlated proteins of PanNEN by proteomics to compare pancreatic tumor (PT) with paired metastatic liver tumor (LT). Of 118 surgical cases with PanNEN, 7 cases with formalin-fixed paraffin-embedded (FFPE) tissues of both PT and paired LT were evaluated by proteomics. Tumor cells were selectively collected from FFPE tissues by laser capture microdissection. A total of 3,722 proteins were detected from extracted peptides by mass spectrometry-based shotgun analysis. Selection of the candidate proteins expressed differently between PT and LT were performed by semi-quantitative comparison in silico and confirmation with immunohistochemistry. We focused on ANXA6, CNPY2, RAB11B and TUBB3, all of which had higher expressions in LT. In all surgical cases with FFPE samples, liver recurrence-free survival (RFS) was evaluated in correlation to the expression of the candidate proteins in PT by immunohistochemistry. Liver RFS was significantly poorer in CNPY2 positive patients than in negative patients (10-year liver RFS; 39.8% vs. 92.3%, p = 0.012). Also, liver RFS tended to be poorer in ANXA6 positive patients than in those who were negative (10-year liver RFS; 51.4% vs. 95.0%, ...Continue Reading

References

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Citations

May 6, 2019·Protein Science : a Publication of the Protein Society·Danny SchildknegtPeter van der Sluijs
May 17, 2019·Expert Review of Proteomics·Laura GiustiAntonio Lucacchini
Dec 6, 2019·Protein and Peptide Letters·Brigida Anna MaioranoAntonio Bianchi

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Methods Mentioned

BETA
GTPase
surgical resection
enzyme-linked immunosorbent assay
deamination
acetylation

Software Mentioned

JMP
Sequest
Mascot

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