A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3.

Molecular Cancer
Xiaoyu HeXianqun Fan

Abstract

Long non-coding RNAs (lncRNAs) have been identified as important epigenetic regulators that play critical roles in human cancers. However, the regulatory functions of lncRNAs in tumorigenesis remains to be elucidated. Here, we aimed to investigate the molecular mechanisms and potential clinical application of a novel lncRNA, retinoblastoma associated transcript-1 (RBAT1), in tumorigenesis. RBAT1 expression was determined by real-time PCR in both retinoblastoma (Rb) and bladder cancer (BCa) cell lines and clinical tissues. Chromatin isolation using RNA purification (ChIRP) assays were performed to identify RBAT1-interacting proteins. Patient-derived xenograft (PDX) retinoblastoma models were established to test the therapeutic potential of RBAT1-targeting GapmeRs. Here, we found that RBAT1 expression was significantly higher in Rb and BCa tissues than that in adjacent tissues. Functional assays revealed that RBAT1 accelerated tumorigenesis both in vitro and in vivo. Mechanistically, RBAT1 recruited HNRNPL protein to E2F3 promoter, thereby activating E2F3 transcription. Therapeutically, GapmeR-mediated RBAT1 silencing significantly inhibited tumorigenesis in orthotopic xenograft retinoblastoma models derived from Rb cell lines an...Continue Reading

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Citations

Jun 28, 2021·Microbial Pathogenesis·Jinfang FengJiujiang He
Aug 6, 2021·Frontiers in Cell and Developmental Biology·Fajuan ChengWei He

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Datasets Mentioned

BETA
OEP000762
GSE111168

Methods Mentioned

BETA
PCR
transfection
immunoprecipitation
RIP
ChIP
Assay
Feature Extraction
xenograft
RNA-Seq
antisense oligonucleotides

Software Mentioned

BD FACSuite
Feature Extraction
ImageJ

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