A Novel Long Non-coding RNA, MSTRG.51053.2 Regulates Cisplatin Resistance by Sponging the miR-432-5p in Non-small Cell Lung Cancer Cells

Frontiers in Oncology
Jie ZhangYufeng Wan

Abstract

Objective: The aim of this study was to investigate the molecular mechanisms underlying cisplatin (DDP) resistance in non-small cell lung cancer (NSCLC) cells by constructing a competing endogenous RNA (ceRNA) network. Methods: The gene expression profiles of human lung adenocarcinoma DDP-resistant cell line (A549/DDP) and its progenitor (A549) were comparatively evaluated by whole-transcriptome sequencing. The differentially expressed genes (DEGs) were subjected to KEGG pathway analysis. The expression levels of mRNAs involved in several pathways associated with conferring DDP resistance to tumor cells were evaluated. The ceRNA network was constructed based on the mRNA expression levels and the sequencing data of miRNA and lncRNA. Several ceRNA regulatory relationships were validated. Results: We quantified the expression of 17 genes involved in the six pathways by quantitative real-time polymerase chain reaction (qRT-PCR). The differential protein expression levels of eight genes were quantified by western blotting. Western blot analysis revealed six differentially expressed proteins (MGST1, MGST3, ABCG2, FXYD2, ALDH3A1, and GST-ω1). Among the genes encoding these six proteins, ABCG2, ALDH3A1, MGST3, and FXYD2 exhibited inter...Continue Reading

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Citations

Sep 10, 2020·Signal Transduction and Targeted Therapy·Huibin SongChang Zou
Jan 19, 2021·Molecular and Cellular Biochemistry·Naira Fernanda Zanchett SchneiderCláudia Maria Oliveira Simões

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Methods Mentioned

BETA
transfection
miRNA-seq
xenografting
RNA Assay
electrophoresis
transfections
Assay
PCR

Software Mentioned

SPSS
ENSEMBL
miRanda
Graphpad Prism
clusterProfiler
HTSeq
cuffdiff
Bowtie
Cufflinks
cuffnorm

Related Concepts