A novel magnetic approach to enhance the efficacy of cell-based gene therapies.

Gene Therapy
M MuthanaC E Lewis

Abstract

Attempts have been made to use various forms of cellular vectors to deliver therapeutic genes to diseased tissues like malignant tumours. However, this approach has proved problematic due to the poor uptake of these vectors by the target tissue. We have devised a novel way of using magnetic nanoparticles (MNPs) to enhance the uptake of such 'therapeutically armed' cells by tumours. Monocytes naturally migrate from the bloodstream into tumours, so attempts have been made to use them to deliver therapeutic genes to these sites. However, transfected monocytes injected systemically fail to infiltrate tumours in large numbers. Using a new in vitro assay for assessing monocyte extravasation, we show that the ability of transfected human monocytes to migrate across a human endothelial cell layer into a 3D tumour spheroid is markedly increased when cells are pre-loaded with MNPs and a magnetic force is applied close to the spheroid. Furthermore, systemic administration of such 'magnetic' monocytes to mice bearing solid tumours led to a marked increase in their extravasation into the tumour in the presence of an external magnet. This new magnetic targeting approach could be used to increase the targeting, and thus the efficacy, of many ...Continue Reading

References

Aug 10, 2000·Nature Biotechnology·D Luo, W M Saltzman
Feb 27, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·S C GoodwinG Wong
May 17, 2003·Cancer Biology & Therapy·Leisha A Emens
Jul 26, 2003·Radiology·Heike E Daldrup-LinkThomas M Link
Mar 17, 2004·Nature Immunology·Alan R SchenkelWilliam A Muller
May 4, 2004·Annals of Neurology·Stasia A AndersonJoseph A Frank
Jul 14, 2004·European Radiology·Stephan MetzHeike E Daldrup-Link
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Benjamin B YellenGary Friedman
Jun 3, 2006·Drugs·Dean M Robinson, Gillian M Keating
Dec 14, 2006·International Journal of Cancer. Journal International Du Cancer·Pauline VerdijkI Jolanda M de Vries
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Craig MurdochClaire E Lewis
Jun 9, 2007·Expert Review of Anticancer Therapy·May D WangShuming Nie

❮ Previous
Next ❯

Citations

Aug 20, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Candice WillmonRichard Vile
Nov 17, 2009·Nature Nanotechnology·Ekaterina I GalanzhaVladimir P Zharov
May 24, 2012·International Journal of Nanomedicine·Michal BabicEva Sykova
May 14, 2010·International Journal of Nanomedicine·Zainulabedin M SaiyedMadhavan P N Nair
Dec 20, 2013·International Journal of Molecular Sciences·Andrea Masotti, Andrea Caporali
Jun 21, 2014·Future Oncology·Parul Benien, Archana Swami
Jul 8, 2014·Therapeutic Delivery·Matthew T BaselDeryl L Troyer
Feb 25, 2009·Expert Opinion on Drug Delivery·Boris Polyak, Gary Friedman
Sep 7, 2011·Advanced Drug Delivery Reviews·Christian PlankOlga Mykhaylyk
Oct 21, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·D G RoyF Le Boeuf
Jan 3, 2013·Journal of Tissue Engineering and Regenerative Medicine·Alicia J El HajJon Dobson
Aug 19, 2015·Nature Communications·Munitta MuthanaClaire Lewis
Nov 9, 2011·Journal of Ethnopharmacology·Craig MurdochMunitta Muthana
Aug 22, 2009·JACC. Cardiovascular Interventions·Panagiotis G KyrtatosMark F Lythgoe
Nov 5, 2013·Biomaterials·Brandon M MattixFrank Alexis
Jan 26, 2010·Journal of Biotechnology·Franziska HirschhaeuserLeoni A Kunz-Schughart
Apr 14, 2016·Experimental Biology and Medicine·Stephanie L HamHossein Tavana
May 25, 2013·International Journal of Molecular Sciences·Anna SchadeGustav Steinhoff
May 28, 2013·International Journal of Molecular Sciences·Cristina RiggioJosé Antonio Del Río
Dec 1, 2009·Nature Materials·Jon Dobson
Apr 14, 2012·Journal of Tissue Engineering and Regenerative Medicine·A FourikiJ Dobson
Oct 24, 2013·Nature Materials·Simona MuraPatrick Couvreur
Dec 24, 2019·Nanomedicine·Faith Howard, Munitta Muthana
Nov 27, 2019·Materials·Esperanza DíazJosé Manuel Barandiarán
May 9, 2012·Oncotarget·Charles LiAndrew C Keates
Jan 16, 2009·Experimental Biology and Medicine·Vincent H B HoNigel K H Slater
Feb 18, 2021·Journal of the Royal Society, Interface·E F YeoA J El Haj
Feb 12, 2017·Pharmacology & Therapeutics·Christopher D ZahmDouglas G McNeel
Jul 25, 2021·International Journal of Molecular Sciences·Francesca Marini, Maria Luisa Brandi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.