A novel mechanism for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta

Scientific Reports
Alyce McClellanDeborah Guest

Abstract

Interleukin 1β (IL-1β) is upregulated following tendon injury. Here we demonstrate that in adult and fetal tenocytes IL-1β increases the expression of matrix metalloproteinases, tenascin-C and Sox9 and decreases the expression of scleraxis and cartilage oligomeric matrix protein. When cultured in 3-dimensional collagen gels adult and fetal tenocytes exposed to IL-1β have reduced contraction ability and generate tendon-like constructs with a lower storage modulus. In contrast, equine embryonic stem cell (ESC) derived tenocytes exposed to IL-1β exhibit no changes in gene expression and generate identical tendon-like constructs. We propose that ESC-derived tenocytes do not respond to IL-1β due to their low expression of interleukin 1 (IL-1) receptor 1 and high expression of the decoy receptor IL-1 receptor 2 and IL-1 receptor antagonist protein (IL1Ra). This may make ESC-derived tenocytes an advantageous source of cells for tissue regeneration and allow the development of novel pharmaceutical interventions to protect endogenous cells from inflammation.

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E BellC Merrill
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R Montesano, L Orci
Nov 6, 1998·Science·J A ThomsonJ M Jones
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 21, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Joanne ArchambaultAlbert J Banes
Nov 7, 2002·The Journal of Veterinary Medical Science·Yoshinao HosakaKazushige Takehana
Feb 6, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M TsuzakiA J Banes
Feb 18, 2003·Journal of Anatomy·Sarah F Oldfield, Darrell J R Evans
Apr 12, 2005·Cardiovascular Research·Olivier D DefaweAlexander W Clowes
Jun 27, 2006·Methods in Molecular Biology·Peter NgoGlenn T Furuta
Sep 1, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michele FavataLouis J Soslowsky
Sep 19, 2006·Stem Cells and Development·Xihe LiW R Allen
Apr 14, 2007·The Journal of Biological Chemistry·Véronique LéjardJerome Rossert
May 24, 2007·Tissue Engineering·Jie QiAlbert J Banes
Feb 12, 2008·Matrix Biology : Journal of the International Society for Matrix Biology·Zoher KapaceeKarl E Kadler
May 10, 2008·Acta Biochimica Et Biophysica Sinica·Yanfang GuoHongwen Deng
Apr 14, 2009·Journal of Molecular and Cellular Cardiology·Leon EspiraMichael P Czubryt
Jan 21, 2010·Science Signaling·Axel WeberMichael Kracht
Feb 24, 2010·Nature Reviews. Rheumatology·Cem GabayGaby Palmer
Aug 26, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Nicholas S KalsonKarl E Kadler
Aug 26, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Zoher KapaceeKarl E Kadler
May 6, 2011·Critical Reviews in Immunology·Irina A UdalovaKim S Midwood
Jun 28, 2011·The Journal of Bone and Joint Surgery. British Volume·S ChaudhuryA J Carr
Aug 30, 2011·The Veterinary Clinics of North America. Equine Practice·Jamie Textor
Sep 2, 2011·Rheumatology International·Christina KaiserBjörn Pilström
Nov 15, 2011·Journal of Shoulder and Elbow Surgery·Salma ChaudhuryAndrew J Carr
Dec 20, 2011·Clinics·Alexandre Leme Godoy-SantosMaria Cristina L G dos Santos
Dec 1, 2012·Brain, Behavior, and Immunity·Vanessa A PetersGregory G Freund
Apr 12, 2013·Journal of Inflammation Research·Qiuhong FangStephen I Rennard
Apr 20, 2013·Journal of Applied Physiology·Andrew S LaCroixRay Vanderby
Feb 22, 2014·Veterinary Immunology and Immunopathology·Stephanie G DakinRoger K W Smith
Apr 8, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tetsu KobayashiStephen I Rennard

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Citations

Mar 18, 2020·Journal of Tissue Engineering and Regenerative Medicine·Francesca AtkinsonDeborah J Guest
Jul 28, 2020·International Journal of Molecular Sciences·Nina FrieseAndrea Hoffmann
Feb 26, 2020·BMC Biomedical Engineering·Sophia K Theodossiou, Nathan R Schiele
Nov 14, 2020·Medicinal Research Reviews·Yuan Tan, Faqin Tang

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Methods Mentioned

BETA
flow cytometry
activated cell sorting
PCR

Software Mentioned

NovoExpress
ImageJ
primer3

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