PMID: 2115866Aug 1, 1990Paper

A novel membrane-associated threonine permease encoded by the tdcC gene of Escherichia coli

Journal of Bacteriology
V N SumantranP Datta

Abstract

A novel L-threonine transport system is induced in Escherichia coli cells when incubated in amino acid-rich medium under anaerobic conditions. Genetic and biochemical analyses with plasmids harboring mutations in the anaerobically expressed tdcABC operon indicated that the tdcC gene product was responsible for L-threonine uptake. Competition experiments revealed that the L-threonine transport system is also involved in L-serine uptake and is partially shared for L-leucine transport; L-alanine, L-valine, and L-isoleucine did not affect L-threonine uptake. Transport of L-threonine was inhibited by the respiratory chain inhibitors KCN and carbonyl cyanide m-chlorophenylhydrazone and was Na+ independent. These results identify for the first time an E. coli gene encoding a permease specific for L-threonine-L-serine transport that is distinct from the previously described threonine-serine transport systems. A two-dimensional topological model predicted from the amino acid composition and hydropathy plot showed that the TdcC polypeptide appears to be an integral membrane protein with several membrane-spanning domains exhibiting a striking similarity with other bacterial permeases.

References

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Citations

Jun 25, 1995·Molecular & General Genetics : MGG·Y Wu, P Datta
Dec 7, 2007·Molecular Systems Biology·Kwang Ho LeeSang Yup Lee
Jun 3, 2010·Biotechnology Journal·Jin Hwan Park, Sang Yup Lee
Jun 10, 2019·Biotechnology and Applied Biochemistry·Zhixiang DingXiaoyuan Wang
Jul 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·John-Demian SauerMichele S Swanson
Jan 25, 2020·Biotechnology and Applied Biochemistry·Lei ZhaoYe Li
Aug 1, 1991·Molecular & General Genetics : MGG·H P Schweizer, P Datta
Apr 24, 2004·The Biochemical Journal·Yang-Hoon KimJeewon Lee
Jul 8, 2005·Microbiology·Y P TanK Y Leung
Dec 1, 2004·EcoSal Plus·George V Stauffer
Nov 1, 2005·EcoSal Plus·Larry Reitzer
Aug 1, 1997·Journal of Bacteriology·S ChattopadhyayP Datta
Dec 1, 1993·Microbiological Reviews·M Riley
Jan 9, 2009·Microbial Cell Factories·Jun Hyoung LeeSun Chang Kim

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