A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase

Enzyme and Microbial Technology
Gang GaoGang Li

Abstract

Functional screening of a metagenomic library of termite hindgut identified an overlapping gene cluster encoding the phosphotransferase system (PTS) components, which consisted of a glucoside specific PTS enzyme II gene (glu1923) and a glycoside hydrolase gene (glu1392). Hydrolytic experiments revealed that the combined effect of Glu1923 and Glu1392 was responsible for the utilization of glucosidic substrates in recombinant Escherichia coli (E. coli) strains. Yeast two hybrid analysis suggested that there was an interaction between Glu1923 and Glu1392, and the domain EIIA of Glu1923 played an important role for the interaction. In addition, the hydrolase Glu1392 displayed hydrolysis ability toward cellobiose and maltose, and had a very high tolerance to glucose with a Ki value of 2.25M. These properties make Glu1392 an interesting candidate in biotechnology applications after further study.

References

Apr 6, 2002·Current Opinion in Microbiology·Jacqueline Plumbridge
Dec 7, 2002·Biotechnology Progress·Sumana Mukherjee, Suman Khowala
Dec 4, 2003·Archives of Microbiology·Barbara SetlowPeter Setlow
Oct 20, 2005·Journal of Bioscience and Bioengineering·P KotrbaH Yukawa
Nov 15, 2006·Applied Microbiology and Biotechnology·Nisha PalackalBrian A Steer
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Dec 22, 2006·Journal of Molecular Microbiology and Biotechnology·Wilfrid J MitchellPhilip G Meaden
May 15, 2007·Journal of Bacteriology·Dragana Ajdić, Vi T T Pham
Nov 22, 2007·Molecular Microbiology·Aashiq H KachrooSubramony Mahadevan
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Dec 31, 2008·Applied and Environmental Microbiology·Karina PokusaevaDouwe van Sinderen
Aug 12, 2009·Applied Biochemistry and Biotechnology·Bruno BenolielLidia Maria Pepe de Moraes
Oct 7, 2010·The EMBO Journal·Livnat LopianOrna Amster-Choder
Jun 30, 2011·Applied Microbiology and Biotechnology·Parisutham Vinuselvi, Sung Kuk Lee
Oct 6, 2012·Journal of Molecular Microbiology and Biotechnology·Konanani RashamuseDean Brady
Oct 9, 2012·Enzyme and Microbial Technology·Qianfu WangZhihua Zhou
May 8, 2013·Applied Microbiology and Biotechnology·Konanani RashamuseDean Brady
May 16, 2013·Biotechnology for Biofuels·Géraldine BastienMichael O'Donohue
Jun 14, 2013·The Journal of General and Applied Microbiology·Cristiane Akemi UchimaManabu Arioka
Jun 10, 2015·Applied Biochemistry and Biotechnology·Tingting FengHaifeng Shi

❮ Previous
Next ❯

Citations

Oct 13, 2016·Frontiers in Microbiology·María-Eugenia DeCastroMaría-Isabel González-Siso
Feb 10, 2018·Scientific Reports·Weihui XuYunpeng Su
May 16, 2017·Biotechnology Letters·Shu ZhangJianwei Wu
Oct 14, 2017·FEMS Microbiology Letters·Francesca BeriniFlavia Marinelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.