PMID: 11507171Aug 17, 2001

A novel method for controlled and reversible long term compression of the umbilical cord in fetal sheep

The Journal of Physiology
D S GardnerD A Giussani

Abstract

1. In fetal sheep during late gestation the aims of the present study were to (1) develop a technique for inducing prolonged but reversible periods of controlled compression of the umbilical cord and (2) characterise the cardiovascular, endocrine and metabolic responses to this challenge. 2. Under 1-2 % halothane anaesthesia, 16 Welsh Mountain sheep fetuses were chronically instrumented at 118 +/- 2 days of gestation (term is ca 145 days) with an inflatable occluder cuff around the umbilical cord, amniotic and femoral vascular catheters and with transit-time flow probes around the contra-lateral femoral artery and an umbilical artery. At 125 days, umbilical blood flow was reduced by 30 % from a pre-determined 24 h baseline for 3 days by automated servo-controlled inflation of the occluder cuff (n = 8). The occluder was then deflated allowing return of umbilical blood flow to baseline. The remaining eight fetuses were used as sham-operated controls in which the occluder was not inflated throughout the protocol. Fetal cardiovascular variables were recorded at 8 s intervals and arterial blood samples taken for measurement of blood gases, glucose and lactate and plasma adrenaline, noradrenaline and vasopressin concentration through...Continue Reading

References

Sep 11, 2003·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Hobe J Schröder
Feb 1, 2010·Journal of Developmental Origins of Health and Disease·C E SalinasD A Giussani
Oct 27, 2015·The Journal of Physiology·D A Giussani
Mar 31, 2004·Journal of the Society for Gynecologic Investigation·Toshiko ImamuraCharles A Ducsay
May 3, 2018·Physiological Reviews·Charles A DucsayLubo Zhang
Apr 28, 2005·American Journal of Physiology. Endocrinology and Metabolism·M G GnanalinghamD S Gardner
Jul 16, 2015·American Journal of Physiology. Endocrinology and Metabolism·Elizabeth A NewbyCharles A Ducsay
Jul 29, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Isa LindgrenJordi Altimiras
Apr 10, 2004·The Journal of Physiology·D S GardnerD A Giussani
Apr 2, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·D S GardnerA L Fowden

Citations

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Related Concepts

Arterial Blood pH Measurement
Metabolic Process, Cellular
Fetal Structures
Salicylhydroxamic acid
Halothane
Lactate
Vascular Resistance
Cardiovascular System
Blood Vessel
Antidiuretic Hormone Measurement

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