A novel method for detection of apoptosis

Experimental Cell Research
A Zagariya

Abstract

There are two different Angiotensin II (ANG II) peptides in nature: Human type (ANG II) and Bovine type (ANG II). These eight amino acid peptides differ only at position 5 where Valine is replaced by Isoleucine in the Bovine type. They are present in all species studied so far. These amino acids are different by only one atom of carbon. This difference is so small, that it will allow any of ANG II, Bovine or Human antibodies to interact with all species and create a universal method for apoptosis detection. ANG II concentrations are found at substantially higher levels in apoptotic, compared to non-apoptotic, tissues. ANG II accumulation can lead to DNA damage, mutations, carcinogenesis and cell death. We demonstrate that Bovine antiserum can be used for universal detection of apoptosis. In 2010, the worldwide market for apoptosis detection reached the $20 billion mark and significantly increases each year. Most commercially available methods are related to Annexin V and TUNNEL. Our new method based on ANG II is more widely known to physicians and scientists compared to previously used methods. Our approach offers a novel alternative for assessing apoptosis activity with enhanced sensitivity, at a lower cost and ease of use.

References

Mar 15, 1997·Experimental Cell Research·E CigolaP Anversa
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael PappBruce D Uhal
Jan 16, 2003·American Journal of Physiology. Renal Physiology·Madhu BhaskaranPravin C Singhal
Sep 11, 2004·Journal of Applied Physiology·Xiaopeng LiBruce D Uhal
Aug 10, 2007·Journal of the American Society of Nephrology : JASN·Anja Sachse, Gunter Wolf
Nov 18, 2008·Cancer Research·Ursula SchmidNicole Schupp

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