PMID: 22571786May 11, 2012Paper

A novel method for determination of drug distribution in rat brain tissue sections by LC/MS/MS: functional tissue microanalysis

Current Topics in Medicinal Chemistry
John A MasucciGary W Caldwell

Abstract

Determination of drug distribution in brain and other tissues is important in pharmaceutical research. Tissue drug levels need to be determined routinely as they are usually diagnostic for both efficacy and toxicity. Determination of tissue levels in small organ subregions is frequently performed due to important functional considerations. These measurements have traditionally been very tedious requiring extensive dissection and specimen pooling to achieve detection of analytes of interest. Direct and indirect methods utilizing mass spectrometry have been reported for detection of analytes in tissue specimens. Typically, these require very specialized MS or sampling equipment and are only partially successful due to analyte response. We have developed a novel approach for quantitation of tissue sections called Functional Tissue Microanalysis (FTM) in which small circular samples are removed from subregions of interest, extracted and analyzed by conventional LC/MS/MS utilizing electrospray ionization. This allows direct measurement of regional concentrations without dissection and homogenization of tissue specimens as many subregions can be sampled from a single mounted section. Utilization of the FTM approach for analysis of bo...Continue Reading

Citations

Mar 31, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Feng-Wei MaChao Zhao
Nov 4, 2020·Analytical and Bioanalytical Chemistry·Vilmos Kertesz, John F Cahill

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.