A novel method for fast and statistically verified morphological characterization of filamentous fungi

Fungal Genetics and Biology : FG & B
Andreas E PoschChristoph Herwig

Abstract

Along with productivity and physiology, morphological growth behavior is the key parameter in bioprocess design for filamentous fungi. Despite complex interactions between fungal morphology, broth viscosity, mixing kinetics, transport characteristics and process productivity, morphology is still commonly tackled only by empirical trial-and-error techniques during strain selection and process development procedures. In fact, morphological growth characteristics are investigated by computational analysis of only a limited number of pre-selected microscopic images or via manual evaluation of images, which causes biased results and does not allow any automation or high-throughput quantification. To overcome the lack of tools for fast, reliable and quantitative morphological analysis, this work introduces a method enabling statistically verified quantification of fungal morphology in accordance with Quality by Design principles. The novel, high-throughput method presented here interlinks fully automated recording of microscopic images with a newly developed evaluation approach reducing the need for manual intervention to a minimum. Validity of results is ensured by concomitantly testing the acquired sample for representativeness by ...Continue Reading

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Citations

Nov 28, 2012·Trends in Biotechnology·Andreas E PoschOliver Spadiut
Sep 6, 2012·Biotechnology and Bioengineering·David J Barry
Mar 29, 2014·Biotechnology and Bioengineering·Difeng GaoShulin Chen
Feb 24, 2018·Applied Microbiology and Biotechnology·Lukas VeiterChristoph Herwig
Jan 23, 2014·BioTechniques·Pekka RuusuvuoriAimée M Dudley
Jul 27, 2016·Engineering in Life Sciences·Alexander GrünbergerStephan Noack
Sep 17, 2017·Antonie van Leeuwenhoek·Joost WillemseGilles P van Wezel
Apr 4, 2018·Bioprocess and Biosystems Engineering·Xiaowen SunZhiming Zheng

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