Abstract
Along with productivity and physiology, morphological growth behavior is the key parameter in bioprocess design for filamentous fungi. Despite complex interactions between fungal morphology, broth viscosity, mixing kinetics, transport characteristics and process productivity, morphology is still commonly tackled only by empirical trial-and-error techniques during strain selection and process development procedures. In fact, morphological growth characteristics are investigated by computational analysis of only a limited number of pre-selected microscopic images or via manual evaluation of images, which causes biased results and does not allow any automation or high-throughput quantification. To overcome the lack of tools for fast, reliable and quantitative morphological analysis, this work introduces a method enabling statistically verified quantification of fungal morphology in accordance with Quality by Design principles. The novel, high-throughput method presented here interlinks fully automated recording of microscopic images with a newly developed evaluation approach reducing the need for manual intervention to a minimum. Validity of results is ensured by concomitantly testing the acquired sample for representativeness by ...Continue Reading
References
Dec 13, 1966·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·S J Pirt
Apr 1, 1999·Biotechnology and Bioengineering·B McNeilS White
Oct 26, 1999·Applied Biochemistry and Biotechnology·H el-EnshasyU Rinas
Apr 19, 2000·Critical Reviews in Biotechnology·P A GibbsF Schmid
Nov 25, 2003·Journal of Applied Microbiology·T L LübbehüsenM McIntyre
Dec 11, 2003·Biotechnology Advances·Maria Papagianni
Jan 18, 2005·Journal of Biotechnology·J L Casas LópezY Chisti
Apr 15, 1990·Biotechnology and Bioengineering·H L Packer, C R Thomas
Mar 12, 2009·Journal of Industrial Microbiology & Biotechnology·David J BarryGwilym A Williams
Apr 22, 2009·Biotechnology and Bioengineering·A DianoJ Nielsen
Jul 14, 2009·Bioresource Technology·Aftab Ahamed, Patrick Vermette
Citations
Jun 26, 2012·Microbial Cell Factories·Andreas E PoschChristoph Herwig
Dec 12, 2012·Fungal Genetics and Biology : FG & B·Andreas E PoschChristoph Herwig
Nov 28, 2012·Trends in Biotechnology·Andreas E PoschOliver Spadiut
Sep 6, 2012·Biotechnology and Bioengineering·David J Barry
Mar 29, 2014·Biotechnology and Bioengineering·Difeng GaoShulin Chen
Mar 13, 2014·Biotechnology Progress·Andreas E Posch, Christoph Herwig
Mar 14, 2014·Rapid Communications in Mass Spectrometry : RCM·Michaela HelmelGünter Allmaier
Dec 3, 2014·Analytical Biochemistry·Michaela HelmelGünter Allmaier
Feb 24, 2018·Applied Microbiology and Biotechnology·Lukas VeiterChristoph Herwig
Jan 23, 2014·BioTechniques·Pekka RuusuvuoriAimée M Dudley
Jun 21, 2019·Applied Microbiology and Biotechnology·Lukas Veiter, Christoph Herwig
Feb 7, 2017·Journal of Microscopy·N HardyF Ben Chaabane
Sep 16, 2017·Applied Microbiology and Biotechnology·Daniela EhgartnerJens Fricke
Apr 25, 2017·Mycobiology·Hanbyul LeeJae-Jin Kim
Jul 27, 2016·Engineering in Life Sciences·Alexander GrünbergerStephan Noack
Sep 17, 2017·Antonie van Leeuwenhoek·Joost WillemseGilles P van Wezel
Apr 4, 2018·Bioprocess and Biosystems Engineering·Xiaowen SunZhiming Zheng
Mar 8, 2021·Fungal biology and biotechnology·Roman JansenMarco Oldiges