A novel method for isolating Schwann cells using the extracellular domain of Necl1.

Journal of Neuroscience Research
Ivo Spiegel, Elior Peles

Abstract

Myelinating cocultures of Schwann cells and dorsal root ganglion neurons are a powerful experimental system for probing the molecular mechanisms of axon-Schwann cell interaction. The isolation of a pure population of myelination-competent Schwann cells is a prerequisite for this experimental system. We describe here a protocol for a FACS-based isolation of Schwann cells utilizing a specific affinity reagent (Necl1-Fc) and the use of these isolated cells in myelinating cocultures. An advantage of the myelinating coculture system is that Schwann cells and the neurons can be genetically manipulated before they are cocultured. We further show that our method allows the isolation of virally transduced Schwann cells in a single purification step. This protocol for the FACS-based isolation of myelination-competent Schwann cells by Necl1-Fc and the use of these cells in myelinating cocultures should significantly facilitate future studies aimed at delineation of the molecular mechanisms of axon-Schwann cell interactions and myelination.

References

Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rakesh NagarajanJeffrey Milbrandt
Feb 28, 2003·Journal of Neuroscience Methods·Jan ManentMarco Giovannini
Dec 20, 2003·Nature Reviews. Neuroscience·Sebastian Poliak, Elior Peles
Sep 1, 2005·Neuron·Carla TaveggiaJames L Salzer
Jun 6, 2007·The Journal of Cell Biology·Yulia DzhashiashviliJames L Salzer
Jun 15, 2007·Nature Neuroscience·Ivo SpiegelElior Peles

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Citations

Jun 10, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Noorjahan B JagalurDies Meijer
Jul 1, 2009·Journal of Neuroscience Research·Menahem EisenbachElior Peles
Jul 30, 2014·Stem Cell Reports·Matthew P KrauseFreda D Miller
Jun 10, 2014·Hearing Research·Sonam DilwaliKonstantina M Stankovic
Jun 20, 2017·Molecular Medicine Reports·Mi ShenFei Ding
Apr 4, 2021·International Journal of Molecular Sciences·Sonia BalestriAntonella Ragnini-Wilson

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